不同浓度酒精对小鼠空间学习记忆能力及海马GFAP/NEUN的影响  

Effects of Different Concentrations of Alcohol On Spatial Learning and Memory Ability and Hippocampal GFAP/NEUN in Mice

作　　者：夏天[1] 袁凤娟[1] 靳兴越 陈惠东[1] 江润益 段盛顺 张家兴[1] 张然[1] 

机构地区：[1]厦门大学医学院基础医学部,福建厦门361000

出　　处：《中国继续医学教育》2017年第10期194-195,共2页China Continuing Medical Education

摘　　要：目的探究长期饮酒对小鼠行为学能力以及空间学习记忆能力的影响。方法雄性KM小鼠45只,随机分成对照组(Con),低剂量组(Low)和高剂量组(High)。分别每天对照组0.10 ml/10 g自来水,低剂量组0.05 ml/10 g的56度红星二锅头,高剂量组0.10 ml/10 g的56度红星二锅头进行灌胃。连续灌胃13 d后进行衣架实验测行为学能力,最后取海马做免疫荧光染色观察海马神经元和星形胶质细胞。结果衣架实验得分,自主活动数值总体显示Low组高于其他两组,而且与Con组差异显著;免疫荧光结果显示无论是CA3区,还是DG区,海马的星形胶质细胞含量与神经元细胞含量比值上Con,Low,High三组依次升高,而且Con,High之间对比,P<0.05,差异有统计学意义。结论长期大量饮酒会损伤学习记忆能力,但适量饮酒可以促进机体兴奋性和活动度。Objective To investigated the effects of long-term alcohol drinking on the ability of learning and memory in mice. Methods 45 male KM mice were randomly divided into control group （Con）, low dose group （Low） and high dose group （High）. The control group were daily 0.10 ml/10 g tap water, low dose group 0.05 ml/10 g, 56 degree star erguotou, high dose group 0.10 ml/10 g, 56 degree star Erguotou by intragastric administration. After 13 days of continuous feeding, the experiment was conducted to test the behavior of the horse＇s tail, and the hippocampus was observed by immunofluorescence staining. Results The hanger score, numerical display Low overall locomotor activity was higher than the other two groups, and group Con; immunofluorescence results showed that both the CA3 region or DG region, astrocytes and neurons of hippocampus content ratio on Con, Low, High three group were significantly increased, and Con, High had significant difference, Conclusion A large number of long-term drinking will damage the ability of learning and memory, but moderate alcohol consumption can promote the body excitability and activity.

关 键 词：红星二锅头 饮酒 GFAP/NEUN 海马 

分 类 号：R33[医药卫生—人体生理学]