合肥地区献血人群HTLV筛查情况及分析  

HTLV screening and analysis in donors in Hefei

作　　者：程卫芳[1] 周学勇[1] 杨斌[1] 丁利[1] 索小红[1] 高德玉[1] 张云[1] 李玲[2] 刘忠[1] 

机构地区：[1]安徽省血液中心,安徽合肥230031 [2]中国医学科学院输血研究所

出　　处：《中国输血杂志》2017年第3期229-230,共2页Chinese Journal of Blood Transfusion

基　　金：协和青年基金(3332015121)

摘　　要：目的调查合肥地区献血者HTLV的血清学流行情况,为该地区血液安全筛查策略的制定提供参考。方法采用国产或进口双抗原夹心法ELISA试剂分别筛查献血者血液标本,初筛反应性标本进行双孔复试,复试反应性标本利用蛋白印迹法(WB)进一步确认。结果 9 964份献血者标本共筛选出4份反应性,占0.04%;经确认3份不确定,1份阴性。结论合肥地区献血人群中存在一定的血清学筛查阳性率,有必要扩大监测量及进行抗-HTLV确认和追踪研究,为是否将HTLV纳入当地献血者血液筛查提供依据。Objective Surveying HTLV epidemiological characteristics of blood donors in Hefei,providing the reference for establishing blood screening strategy formulation.Methods Donors samples were tested by an ELISA reagents which was made in China or exported.All samples of ELISA screening reactive were retested twice,if retested results were still reavtive,the samples was confirmedby Westen Blot （WB）.Results There were 9 964 samples were collected and tested by ELISA reagents,of these,4 donors samples were screening reactive （0.04%）.These samples were confirmed by WB,3 donors were determined indeterminate,1 donors was determined false positives.Conclusion According to the experimental results,there were certain screening positive rate of donors in Hefei.It is necessary to expand the amount of monitoring and follow-up,providing the reference for deciding whether screening HTLV-Ⅰ / Ⅱ antibodyin blood donors.

关 键 词：献血者 HTLV-Ⅰ/Ⅱ ELISA 筛查 合肥地区 

分 类 号：R373[医药卫生—病原生物学] R446.11[医药卫生—基础医学]