四川地区冠心病患者CYP2C19基因多态性检测  

Genetic polymorphism analysis of CYP2C19 in patients with coronary heart disease in Sichuan Province

作　　者：曾敏[1,2] 熊怡淞[1] 俞娟[3] 常凯[1] 吴艾霖[1] 

机构地区：[1]中国人民解放军成都军区总医院检验科,610083 [2]成都医学院检验医学院,610500 [3]南通大学附属医院检验科,江苏南通226001

出　　处：《检验医学与临床》2017年第10期1363-1365,共3页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81302596,81301484);成都军区总医院院管课题(2013YG-B064)

摘　　要：目的检测四川地区冠心病患者CYP2C19基因的多态性,分析基因型与性别、年龄及临床分型之间的相关性。方法采用DNA微阵列芯片法检测131例被确诊为冠心病患者的CYP2C19基因型,结合患者的性别、年龄、临床分型进行分组,探讨这些因素与CYP2C19基因多态性的相关性。结果 131例冠心病患者中,有5种基因型被检测到,分别为CYP2C19*1/*1(636GG,681GG)、CYP2C19*1/*2(636GG,681GA)、CYP2C19*1/*3(636GA,681GG)、CYP2C19*2/*2(636GG,681AA)、CYP2C19*2/*3(636GA,681GA)。分布频率分别为53例(40.5%)、54例(41.2%)、6例(4.6%)、13例(9.9%)、5例(3.8%),而CYP2C19*3/*3(636AA,681GG)未被检测到。不同性别冠心病患者CYP2C19的基因型、等位基因频率差异无统计学意义(P>0.05);不同性别的代谢型患者,其CYP2C19的基因型分布差异具有统计学意义(χ~2=6.527,P=0.038)。不同年龄和临床分型冠心病患者CYP2C19的基因型、等位基因频率、代谢型分布差异无统计学意义(P<0.05)。结论冠心病患者的CYP2C19基因型分布、等位基因频率分布与性别、年龄和疾病临床分型无关。Objective To detect the CYP2C19 gene polymorphisms in patients with coronary heart disease（CHD）in Sichuan Province,and to analyze the correlation of its genotype with gender,age and clinical classification.Methods By DNA microarray chip,CYP2C19 gene type of total 131 cases,including 90 males and 41 females,diagnosed with CHD were detected.The correlation of CYP2C19 gene polymorphisms with gender,age and clinical classification was statistically analyzed.Results In the 131 cases of CHD patients,five genotypes were detected,including CYP2C19＊1/＊1（636GG,681GG）,CYP2C19＊1/＊2（636GG,681GA）,CYP2C19＊1/＊3（636GA,681GG）,CYP2C19＊2/＊2（636GG,681AA）and CYP2C19＊2/＊3（636GA,681GA）.The distribution of genotypes were 53cases（40.5%）,54cases（41.2%）,6cases（4.6%）,13cases（9.9%）and 5cases（3.8%）,respectively.CYP2C19＊3/＊3（636AA,681GG）was not detected.The CYP2C19 genotypes and allele frequency between different genders had no statistical difference in CHD patients（P〈0.05）,while CYP2C19 metabolic type distributions between male and female CHD patients had significant difference（χ^2=6.527,P=0.038）.Different age groups and different CHD clinical sub-type patients had no statistical difference in CYP2C19 genotypes,allele frequency and metabolic type distribution（P〈0.05）,respectively.ConclusionCYP2C19 genotype distribution,allele frequency and metabolic type distribution among the CHD patients have no correlation with genders,age and CHD clinical sub-types.

关 键 词：CYP2C19 冠心病 基因芯片 基因多态性 

分 类 号：R971.6[医药卫生—药品]