EC-SOD在肾缺血再灌注损伤大鼠脑内的表达变化  

Expression changes of EC-SOD in the brain of the renal ischemia reperfusion injury in rats

作　　者：郝斌[1] 王切[2] 王素玲[3] 王磊[2] 

机构地区：[1]河北省沧州市中心医院泌尿外科,河北沧州061001 [2]河北医科大学基础医学院人体解剖学教研室,河北石家庄050017 [3]河北省血液中心检验科,河北石家庄050071

出　　处：《河北医科大学学报》2017年第5期552-556,共5页Journal of Hebei Medical University

基　　金：河北省医学科学研究重点课题(20160088)

摘　　要：目的观察细胞外超氧化物歧化酶(extracellular superoxide dismutases,EC-SOD)在肾缺血再灌注损伤(renal ischemia reperfusion injury,RIRI)大鼠脑内的表达变化,探讨EC-SOD在脑抗氧化应激反应中的作用。方法选用健康雄性Wistar大鼠12只,随机分为对照组和RIRI组各6只,RIRI组切除大鼠右肾后,用无损伤动脉夹夹闭大鼠左肾动脉,45min后移去动脉夹,恢复大鼠左肾血液供应,建立大鼠肾缺血再灌注损伤模型。待左肾动脉再灌注24h后麻醉大鼠取材(血、肾、脑)。对照组大鼠只分离左肾动脉并不夹闭。选用苦味酸和酶偶联速率法,检测2组大鼠血清中血尿素氮(blood urea nitrogen,BUN)、血清肌酐(serum creatinine,SCr)浓度;苏木精-伊红染色光镜观察实验大鼠肾组织形态变化;采用硫代巴比妥酸比色法检测2组大鼠脑内丙二醛(malondialdehyde,MDA)含量;应用逆转录聚合酶链反应和western blot法,测定大鼠脑内EC-SOD mRNA与蛋白的表达水平。结果RIRI组大鼠血清中BUN和SCr浓度明显高于对照组,RIRI组大鼠脑内MDA含量、H2O2浓度、EC-SOD mRNA水平相对表达量以及EC-SOD蛋白水平相对表达量均高于对照组,差异有统计学意义(P<0.05)。RIRI组脑组织MDA、H2O2含量与EC-SOD mRNA水平以及EC-SOD蛋白水平表达呈正相关。结论大鼠RIRI后,脑内出现过氧化损伤;EC-SOD在RIRI大鼠脑内的表达升高,可能发挥了抗氧化应激的作用。Objective To observe the expression of extracellular superoxide dismutases（EC- SOD） in renal ischemia reperfusion injury（RIRI） rats and to investigate the role of EC-SOD in the brain oxidative stress response. Methods The male Wistar rat were divided randomly into RIRI group and control group ,6 rats in each group. After the right kidney was removed in RIRI rats, the left renal artery was clamped with a non injured artery clamp. After 45 min, the rats were removed and the blood supply was restored. After 24 hours of reperfusion, the blood, kidney and brain were taken. The left renal artery were only separated, but not cliped in the control group rats. The urea nitrogen（BUN） and serum creatinine（SCr） in the serum blood of two groups were detected by picric acid method and enzyme coupling rate method. The malondialdehyde （MDA）content in the brain tissue of two groups was determined by thiobarbituric acid colorimetric method, the renal morphological change was observed by HE staining. The EC-SOD mRNA and protein expression in brain were evaluated by reverse transcription polymerase chain reaction and western blot. Results Compared with the control group, the BUN and SCr in serum of RIRI group were significantly increased. The content of MDA, H2O2, the relative expression level of EC-SOD and the relative expression level of EC- SOD protein in RIRI group were higher than those in control group. The difference was statistically significant（P〈0.05）. The content of MDA and H2O2 was positively correlated with the expression of EC-SOD mRNA and protein in the brain tissue of RIRI group. Conclusion After the RIRI, oxidative damage in the brain was occured. The increase of EC-SOD expression in the brain of RIRI rats may play an important role in anti oxidative stress.

关 键 词：再灌注损伤 超氧化物歧化酶 丙二醛 

分 类 号：R619.9[医药卫生—外科学]