亚慢性Sm(NO_3)_3暴露对小鼠肝脏的影响  

Effects of Subchronic Exposure of Samarium Nitrate on Mice Liver

作　　者：阮琴[1,2] 申秀英[3] 饶和平[4] 王芳[1] 郑绍成[1] 徐晓虹[5] 俞世钢[1] 姜科声[5] Ruan Qin Shen Xiuying Rao Heping Wang Fang Zheng Shaocheng Xu Xiaohong Yu Shigang Jiang Kesheng(School of Xingzhi, Zhejiang Normal University, Jinhua 321004, China Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China College of Biology and Chemical Engineering, Zhefiang University of Science and Technology, Hangzhou 321003, China Quzhou Technical College School of Medicine, Quzhou 324000, China College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China)

机构地区：[1]浙江师范大学行知学院,浙江金华321004 [2]浙江省野生动物生物技术与保护利用重点实验室,浙江金华321004 [3]浙江科技学院生物与化学工程学院,浙江杭州310023 [4]衢州职业技术学院医学院,浙江衢州324000 [5]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《中国稀土学报》2017年第3期383-391,共9页Journal of the Chinese Society of Rare Earths

基　　金：浙江省教育厅项目(Y201329454);浙江省自然科学基金项目(Y5110135)资助

摘　　要：探讨Sm(NO_3)_3亚慢性暴露对小鼠肝功能、肝脏组织结构、酶活的影响。ICR小鼠80只,雌雄各半,随机分成5组,分别自由饮用0,5,50,500,2000 mg·L^(-1)的Sm(NO_3)_3溶液90 d,测定小鼠肝功能指标及肝脏组织SOD,GSH-Px,CAT活力及MDA含量,肝脏光镜切片观察肝组织形态学的变化。结果显示60,90 d 2000 mg·L^(-1)雌性组体重、雄性组肝脏脏器系数与对照组相比具有显著下降(P<0.05);2000 mg·L^(-1)AST雌雄性组、ALT雄性组、Urea雌性组与对照相比具有显著升高(P<0.05),ALB雄性组与对照相比显著降低(P<0.05);GSH-PX活力5,50 mg·L^(-1)雄性组与对照组相比有极显著升高(P<0.01),而500,2000 mg·L^(-1)组雄性鼠有极显著和显著下降(P<0.01,P<0.05),SOD活力在500,2000 mg·L^(-1)雌雄组与对照组相比有显著和极显著降低(P<0.05,P<0.01),MDA在50,500,2000 mg·L^(-1)雌性组与对照相比有显著与极显著升高(P<0.05,P<0.01),CAT 500,2000 mg·L^(-1)雄性组与对照组相比有显著降低(P<0.05);500 mg·L^(-1)组肝细胞肿大,胞质疏松,肝窦、中央静脉有狭窄,2000 mg·L^(-1)细胞排列紊乱,细胞界限不清,部分肝窦狭小甚至完全闭塞,少数肝细胞有破裂现象。以上结果表明亚慢性Sm(NO_3)_3暴露可导致小鼠肝功能异常、肝组织结构损伤,并与暴露浓度、时间及性别相关;提示氧化损伤可能是亚慢性Sm(NO_3)_3暴露致小鼠肝脏毒性损伤的作用机制之一。The effects of sub-chronic exposure to Sm（ NO3 ）3 on function, structure, and enzyme activities of liver in mice were studied. ICR mice were randomly divided into 5 groups （n = 16 ） , after drinking water containing Sm（NO3 ）3 at 0, 5, 50, 500, 2000 mg·L^-1 for 90 d, the function and levels of SOD, GSH Px, CAT activity, and MDA of liver were tested, the changes of liver tissue morphology were observed by light microscope. The results showed that body weight and liver index significantly decreased in the male and female groups following Sin（ NO3 ）3 exposed at 2000 mg·L^-1 for 60 and 90 d （ P 〈 0.05 ）. After exposed to Sm（ NO3 ） 3 at 2000 mg·L^-1, AST and ALT in males and urea in females significantly increased （ P 〈 0.05 ）, but ALB in males significantly decreased （ P 〈 0. 05） when compared to the controls. GSH-PX significantly increased （P 〈0.01 ） in 5 and 50mg·L^-1 Sm（ NO3 ）3 but decreased in 500 and 2000mg·L^-1 Sm（NO3 ）3 in males （P 〈0. 01, P 〈0. 05）. SOD in 500 and 2000 mg.L-1 males and females （P 〈 0.05, P 〈 0.01 ） and CAT in 500 and 2000mg·L^-1 males （ P 〈 0. 05 ） significantly decreased, but MDA in 50, 500, and 2000mg·L^-1 females significantly increased （P 〈0. 05, P 〈0.01 ）. The results of Morphology of liver showed that liver cells became swelling, cytoplasmic was loose, cell boundary was not clear, hepatic sinus and central vein became narrow, and some liver cells were break after exposure to Sm （NO3 ）3 at 500 or 2000mg·L^-1. These results suggested that sub-chronic exposure to Sm（ NO3 ）3 lead to abnormal function and damage of liver cells, which may be associated with oxidative damage induced by exposure subchronic to Sm （ NO3 ） 3.

关 键 词：Sm(NO3)3 亚慢性暴露 肝功能 SOD GSH-PX CAT MDA 肝组织结构 

分 类 号：R994.6[医药卫生—毒理学] R114[医药卫生—药学]