不同产地和不同采收期辽东楤木叶中两种皂苷的含量测定  

Determination of Two Saponins in Aralia elata Leaves from Different Habitats and Gathering Periods

作　　者：李百美 孙宜春[1] 王秋红[1] 王知斌[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学,教育部北药基础与应用研究重点实验室,哈尔滨150040

出　　处：《中国实验方剂学杂志》2017年第11期59-62,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2011ZX09102-001-18)

摘　　要：目的:测定并比较不同产地和不同采收期辽东楤木叶中3-O-β-D-葡萄吡喃糖基-(1→3)-β-D-葡萄吡喃糖基-(1→3)-β-D-葡萄吡喃糖基-齐墩果酸-28-O-β-D-吡喃葡萄糖苷(楤木皂苷TTP)和3-O-[β-D-葡萄吡喃糖基(1→2)]-[β-D-葡萄吡喃糖基(1→3)]-β-D-葡萄吡喃糖基-齐墩果酸-28-O-β-D-葡萄吡喃糖酯(楤木皂苷V)的含量。方法:采用高效液相色谱蒸发光散射检测(HPLC-ELSD)法,使用Diamonsil C_(18)(2)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(31∶69),流速1.0 m L·min^(-1),柱温30℃,蒸发光散射检测器漂移管温度110℃,载气(N2),流速3.2 m L·min^(-1)。结果:楤木皂苷TTP和楤木皂苷V分别在进样量0.51~2.55μg和0.50~4.00μg线性关系良好,相关系数分别为0.999 8和0.999 2。结果表明,不同产地和不同采收期辽东楤木叶中楤木皂苷TTP和楤木皂苷V的含量有所差异,可能与辽东楤木分布区域、生长季节及叶片生理状态有关,由实验结果可以看出,两种皂苷类成分大多在7,8月的含量相对较高,部分地区9月含量较高。结论:该方法简单、快捷、准确,可以同时测定辽东楤木叶中楤木皂苷TTP和楤木皂苷V的含量,为辽东楤木叶质量控制提供实验依据。Objective： To determine and compare the contents of congmunoside TTP and congmunoside V in Aralia elata leaves from different habitats and gathering periods. Method： HPLC-ELSD was carried out on a Diamonsil C18 （2） column （4.6 mm ×250 mm, 5 μm） at a column temperature of 30℃. The mobile phase was acetonitrile and water （31 ： 69） at flow rate of 1.0 mL-min-l. The detector was ELSD, with the temperature of drift tube set at 110℃ , and the flow rate of carrier gas （N2） was 3.2 mL.min-1. Result： The linear ranges of congmunoside TTP and congmunoside V were 0.51-2.55 μg （r =0.999 8） and 0.50-4.00 μg （r =0. 999 2）, respectively. According to the results, the contents of congmunoside TTP and congmunoside V in A. elata leaves from different habitats and gathering periods were different. This may be related to distribution areas, growing seasons and physiological state of leaves. On the basis of the experimental results, the content of two kinds of ingredients was high mostly in July and August, and partially in September. Conclusion： The method is simple, rapid and accurate for determining simultaneously the contents of congmunoside TTP and congmunoside V in A. elata leaves, and can provide a basis for the quality control of A. elata leaves.

关 键 词：高效液相色谱-蒸发光检测 辽东楤木叶 楤木皂苷TTP 楤木皂苷V 含量测定 

分 类 号：R284.1[医药卫生—中药学]