乌药不同部位乌药醚内酯、去甲异波尔定含量比较和FT-NIR光谱分析  

Comparison on Content of Linderane,Norisoboldine in Different Parts of Linderae Radix and FT-NIR Analysis

作　　者：吴瑶[1,2] 岳显可[1,2] 葛卫红[1,2] 杨柳[2] 杜伟锋[1,2] 陆兔林[3] 王智民[4] 

机构地区：[1]浙江中医药大学中药炮制技术研究中心,杭州311401 [2]浙江中医药大学中药饮片有限公司,杭州311041 [3]南京中医药大学药学院,南京210046 [4]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2017年第11期73-78,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业科研专项(201507002)

摘　　要：目的:检测乌药不同部位乌药醚内酯和去甲异波尔定含量,并对FT-NIR光谱进行分析,为乌药产地炮制加工提供资料。方法:将乌药分为表皮组、去皮组和原药组,以2015年版《中国药典》中乌药项下乌药醚内酯和去甲异波尔定色谱条件,对乌药不同部位乌药醚内酯和去甲异波尔定含量进行测定;选取乌药表皮组、去皮组和原药组的近红外光谱图,运用主成分分析-马氏距离进行判别分析,选择谱段5 053.85~4 115.54 cm-1,采用"标准正则变换+原始光谱+一阶求导"组合对原始光谱进行处理,建立主成分分析模型。结果:FT-NIR判别分析模型将乌药3个不同部位清晰地聚为三类,界限明显;HPLC分析结果表明乌药不同部位乌药醚内酯和去甲异波尔定含量为表皮组>原药组>去皮组。结论:HPLC分析结果表明,乌药表皮中乌药醚内酯和去甲异波尔定含量较高,去皮组最低;FT-NIR光谱进行分析进一步证明了乌药不同部位的质量差异,二者共同为指导乌药在加工过程中"去皮与否"提供科学依据。Objective： To examine the content of linderane and norisoboldine in different parts of Linderae Radix, and make a discriminant analysis by fourier transformation infrared spectroscopy. Method： Linderae Radix was divided into three groups, namely skin group, peeling group and original group. According to the chromatographic conditions for linderane and norisoboldine of Linderae Radix in Chinese Pharmacopoeia, HPLC was used to examine the content of linderane and norisoboldine in different parts of Linderae Radix. Near-infrared spectra of skin group, peeling group and original group samples were analyzed through principal component analysis. The principal components analysis model was developed based on the spectrum band of 5 053.85- 4 115.54 cm -1 with ＇ SNV ＋ spectrum ＋ F-G＇. Result： Three samples from the three parts of Linderae Radix were divided into three categories by the model of FT-NIR qualitative discriminant analysis, and there was a clear boundary between the different parts. According to the findings of HPLC analysis, the sequence of the content of linderane and norisoboldine in different parts from high to low was skin group 〉 original group 〉 peeling group. Conclusion ： The results of HPLC analysis showed that the content of linderane and norisoboldine in Linderae Radix skin group was higher, and that of the peeling group was the lowest. FT-NIR analysis further proved the differences in the quality of different part Linderae Radix. The two analysis methods provide the scientific basis for weather Linderae Radix shall be peeled or not during processing.

关 键 词：乌药 主成分分析 产地炮制加工 乌药醚内酯 去甲异波尔定 

分 类 号：R284.1[医药卫生—中药学]