HPLC波长切换法同时测定广藿香配方颗粒中咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和广藿香酮的含量  

Simultaneous Determination of Caffeic Acid,Acteoside,Isoacteoside and Pogostone in Pogostemonis Herba Formula Granules by HPLC with Changing Wavelength

作　　者：甄亚钦[1,2] 田伟[1,2] 王迎春[1,2] 姜国志 牛丽颖[1,2] 

机构地区：[1]河北中医学院,石家庄050091 [2]河北省中药配方颗粒工程技术研究中心河北省高校中药配方颗粒应用技术研发中心,石家庄050091 [3]神威药业集团有限公司,石家庄051430

出　　处：《中国实验方剂学杂志》2017年第11期79-83,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省高等学校科学技术研究项目(ZD2015001)

摘　　要：目的:建立广藿香配方颗粒中咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和广藿香酮的含量测定方法,为其质量标准的研究提供依据。方法:采用高效液相色谱法。Wondasil C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸溶液梯度洗脱,流速1.0 m L·min^(-1),检测波长分别为325 nm(0~18 min,咖啡酸),330 nm(18~30 min,毛蕊花糖苷、异毛蕊花糖苷),311 nm(30~55 min,广藿香酮),柱温30℃。结果:广藿香配方颗粒中咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和广藿香酮进样量分别在0.021 8~0.218μg(r=0.999 8),0.032 6~0.326μg(r=0.999 8),0.099 4~0.994μg(r=0.999 7),0.174 2~1.742μg(r=0.999 9)与峰面积呈良好的线性关系,供试品溶液中4种被测指标成分在24 h内稳定性良好,平均加样回收率分别为98.85%,99.53%,99.37%,100.58%,RSD分别为1.9%,1.1%,1.4%,2.0%。结论:该方法同时测定了广藿香配方颗粒中非挥发性成分咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和挥发性成分广藿香酮的含量,方法可行,重复性好,准确度高,可以为广藿香配方颗粒的质量控制提供方法参考。Objective： To establish a HPLC method to simultaneously determine the content of caffeic acid, acteoside, isoacteoside and pogostone in Pogostemonis Herba formula granules, in order to provide basis for studying its quality standards. Method： An HPLC method was developed. Wondasil C18 column （4.6 mm × 250 mm, 5 μm） was used and eluted with mobile gradient of 0. 1% phosphoric acid-acetonitrile at the flow rate of 1.0 mL＇min-1. The wavelength were set at 325 nm （0-18 min, caffeic acid）, 330 nm（ 18-30 min, acteoside and isoacteoside） , and 311 nm （30-55 min, pogostone）. The column temperature was 30 ℃. Result： The good linear relationships between the concentration and peak area were between O. 021 8-0. 218 txg （r = 0. 999 8） for caffeic acid, 0. 032 6-0. 326 Ixg （r =0. 999 8） for acteoside, 0. 099 4-0. 994 ixg （r =0. 999 7） for isoaeteoside acid and 0. 174 2-1. 742 I^g for pogostone （r = 0. 999 9） , respectively. The four tested indicators of sample solutions were steady within 24 h. The average recoveries were 98.85% , 99.53% , 99.37% , 100.58% , with RSD of 1.9% , 1. 1% , 1.4% , 2.0% , respectively. Conclusion： Caffeic acid, acteoside, isoacteoside and pogostone were determined by this method simultaneously. The method is feasible and accurate with a good reproducibility, and can provide a reference for quality control.

关 键 词：广藿香配方颗粒 波长切换 咖啡酸 毛蕊花糖苷 异毛蕊花糖苷 广藿香酮 

分 类 号：R284.1[医药卫生—中药学]