宫颈癌发生与血浆RelB和PSMD10蛋白质表达调控的关系及临床意义  

Association of Cervical Cancer Carcinogenesis with the Regulation of Plasma RelB and PSMD10 Protein Expression and Clinical Outcome

作　　者：玛依拉.卡米力江 哈丽丹.热依木 阿丽叶古丽.艾皮热木 伊力努尔.沙比提 Abulizi Abudula 

机构地区：[1]新疆维吾尔自治区人民医院,乌鲁木齐830011 [2]新疆医科大学第二附属医院 [3]新疆医科大学附属肿瘤医院 [4]新疆医科大学2011协同创新中心重点实验室

出　　处：《医学研究杂志》2017年第5期28-31,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81360321)

摘　　要：目的在前期蛋白质组学研究中,明确了一系列候选血浆差异蛋白指标,包括Rel B、PSMD10等含量上调可能是宫颈癌的候选血浆蛋白质指标。本研究对上述指标进行定量验证,探讨宫颈癌发生及进展与血浆蛋白质表达调控的关系及临床意义。方法收集维吾尔族女性宫颈病变患者外周血标本共70例,其中宫颈炎(cervicitis,CV)20例,宫颈内上皮瘤变(cervical intraepithelial neoplasisa,CIN)Ⅱ~Ⅲ20例及宫颈鳞癌(cervical squamous cell carcinoma,CSCC)30例,采用酶联免疫法对血浆Rel B、PSMD10表达水平进行定量鉴定。结果宫颈病变进展伴有血浆Rel B、PSMD10蛋白质含量梯度性上调,其差异有统计学意义(P<0.05)。通过不同血浆蛋白质含量变化的相关性分析,发现血浆PSMD10与Rel B呈低度正相关(r<0.4,P<0.01)。对不同蛋白质指标的检测对宫颈癌及癌前病变的预测能力分析,发现血浆Rel B蛋白质检测的敏感度、特异性和准确度较高(依次为90.0%、50.0%和75.7%),对宫颈癌诊断可能有较高的预测价值。结论宫颈癌发生与血浆蛋白质表达调控存在密切关系,血浆Rel B、PSMD10含量上调可能是宫颈癌发生的早期预警的血浆检测指标,对其检测对宫颈癌诊断具有较高的预测价值。Objective In previous proteomics studies, we identified a series of candidate plasma protein markers including RelB and PSMD10. The up regulation of those protein levels may have a causal relationship with the development of cervical cancer. In this study, we made quantitative detection of those two candidate plasma protein markers and discuss the association of cervical cancer carcinogenesis with the regulation of plasma protein expression and clinical outcome. Methods We collected plasma samples of seventy cases from Uyghur women with cervicitis （CV）, cervical intraepithelial neoplasia （CIN） Ⅱ-Ⅲ and cervical squamous cell carcinoma （CSCC）. The content of plasma proteins RelB and PSMD10 was quantitatively determined by enzyme linked immunosorbent assay （ELISA）. Results The development of cervical lesion was accompanied with the gradual increase in RelB and PSMD10 with significant differences （P〈0.05）. Correlation analysis of cervical cancer-specific protein indicators showed that PSMD10 and RelB had a low positive correlation exists at the level of protein expression with significant（r〈0.4,P〈0.01）. Analysis the different protein indicators＇ predictive ability for cervical cancer and precancerous lesions in the sensitivity, specificity, and accuracy of detection showed that RelB had higher sensitivity,specificity and accuracy（90.0%,50.0%,75.7%） than PSMD10. Conclusion Cervical cancer carcinogenesis has a close association with the regulation of plasma protein. The up-regulated plasma level of RelB, PSMD10 was closely associated with cervical carcinogenesis. These findings provide evidence for these proteins as plasma-based biomarkers for early detection of the cervical carcinoma.

关 键 词：宫颈癌 酶联免疫吸附试验 血浆蛋白标志物 

分 类 号：R34[医药卫生—基础医学]