深色果蔬维生素C检测方法的优化与比较  

Optimization and Comparison of Detection Methods of Vitamin C in Dark Fruits and Vegetables

作　　者：盖琼辉[1] 王春林[1] 

机构地区：[1]陇东学院,甘肃庆阳745000

出　　处：《中国食物与营养》2017年第4期38-42,共5页Food and Nutrition in China

摘　　要：目的:优化2,6-二氯靛酚钠滴定法和高效液相色谱法检测维生素C含量操作规范性及适用性。方法:对检测条件的设置、试剂及样液的保存时间、提取剂及浓度的选择、提取过程、脱色剂用量及2种检测方法的精密度、重复性和加标回收率等关键影响因素进行比较。结果:高效液相色谱法检测设置C18(5μm,4.6×250mm)色谱柱,0.1%草酸为流动相,流速1m L/min,柱温30℃,检测波长254nm,所得图谱保留时间适宜,峰形良好,维生素C标准液浓度在0.025~0.3mg/m L范围内线性关系良好,回归方程为:y=46 116x+173.26,R2为0.999 2;维生素C标准液在4~6℃保存下48h内可用、2,6-二氯靛酚钠溶液在4~6℃保存下应在12d内使用,待测液制备完成后应立即测定,若无法立即测定,可于4~6℃冰箱中保存1.5h;2%草酸为提取剂效果最佳,样品加提取液匀浆后迅速定容、过滤和检测,不可久置;使用2,6-二氯靛酚钠滴定法时需添加0.4g/30m L的活性炭进行脱色;精密度、重复性及加标回收率均符合测定要求。结论:2种检测方法都适用于深色果蔬维生素C含量检测,各有特点,可根据测定要求及实际条件选择适宜的方法。[Objective] To optimize standardization and applicability of 2,6-DCIP titration method and HPLC method to determine content of vitamin C. [Method] We compared the key influencing factors of setting, detecting storage time of reagent and sample solution, the extracting agent and the concentration of selection, extraction process, bleaching agent and two kinds of detection methods of precision, repeatability and recoveries. [Result] Vitamin C standard solution stored at 4℃ no more than 48h, 2,6-dichloro indophenol solution stored at 4℃ no more than 12d, and sample extracts store at 4℃ no more than 1.5h, there was no significant change. The extraction test results showed that 2% oxalic acid solution was the best, and the extraction process were add the extract homogenate into sample agent, then constant volume, filter, and detect rapidly. In 2,6-dichloro indophenol method, the sample liquid from deep color samples for determination on vitamin C needed decolorizing by adding 0. 4g/30mL active carbon. [Conclusion] Two detection methods are all suitable for the detection of vitamin C content in dark fruits and vegetables, each has its characteristics, which can be selected according to the requirements of the test and the actual conditions.

关 键 词：深色果蔬 草莓 维生素C 高效液相色谱 2 6-二氯靛酚钠 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]