小麦-滨麦2D/2Ns异代换系DM2411的分子细胞遗传学鉴定  

Molecular Cytogenetic and Morphological Identification of a Wheat-Leymus mollis 2Ns (2D) Substitution Line DM2411

作　　者：刘洋[1] 赵继新[1] 谈宏斌 王秀娟[1] 李家创 梁邦平[1] 刁慧珊 白宇皓 武军[1] 陈新宏[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100 [2]陕西省种业集团有限责任公司,陕西杨凌712100

出　　处：《麦类作物学报》2017年第5期571-577,共7页Journal of Triticeae Crops

基　　金：陕西省自然科学基金项目(2015JM3095);西北农林科技大学唐仲英育种基金项目

摘　　要：为进一步挖掘利用滨麦优异基因,并丰富小麦遗传种质资源,利用形态学、细胞遗传学、基因组原位杂交(Genomic in situ hybridization,GISH)、EST-STS分子标记、SSR分子标记等技术,对从八倍体小滨麦M842-16和硬粒小麦D4286杂交F_7代材料中筛选出的1个遗传稳定的小滨麦异代换系DM2411进行了鉴定。细胞遗传学观察表明,DM2411的染色体主要构型为2n=42=21Ⅱ,遗传稳定。根尖体细胞和花粉母细胞的原位杂交研究表明,DM2411含有1对滨麦Ns基因组。SSR分析表明,DM2411可能缺失了小麦2D染色体。EST分析表明,DM2411可能含有滨麦2Ns染色体。形态学调查表明,DM2411的株高极显著降低。A wheat-L, mollis alien substitution line DM2411 was identified from the F7 progenies of oc- toploid Tritileymus M842-16 and T. durum D4286 by using morphology,cytogenetics,genomic in situ hybridization （GISH）, expressed sequence tag-sequence tagged site（EST-STS） and simple sequence repeat （SSR）. Cytological studies demonstrated that DM2411 had a chromosome karyotype of 2n=42 =21 Ⅱ. GISH analysis indicated that DM2411 had a pair of Ns chromosomes from L. mollis. Analysis with SSR and EST-STS markers showed that the wheat 2Ds chromosomes were substituted by a pair of 2Ns chromosomes from L. mollis in DM2411. Agronomic trait evaluations showed that the plant height of DM2411 was dramatically lowered. Therefore, the substitution line DM2411 was a dwarf material, which could be exploited as an intermediate material in wheat breeding programs and genetic resources.

关 键 词：小麦 滨麦 代换系 GISH EST-STS SSR 

分 类 号：S512.1[农业科学—作物学] S330