三维打印制备掺铁介孔硅酸钙/3-羟基丁酸-3-羟基己酸共聚酯复合支架  

Three-dimensional printing of Fe-containing mesoporous calcium-silicate/poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) composite scaffolds

作　　者：张旭[1] 吴良浩[1] 李得见 敖荣广[1] 陈帆成 禹宝庆[1] 

机构地区：[1]复旦大学附属浦东医院骨科,上海市200120

出　　处：《中国组织工程研究》2017年第14期2140-2146,共7页Chinese Journal of Tissue Engineering Research

基　　金：上海市科委基础研究领域项目(13JC1407302);浦东新区卫生系统重点学科群建设资助(PWZxq2014-03);上海市卫生局重点课题(20134039)~~

摘　　要：背景:由于三维打印技术便捷、快速、操控性强的突出优点及可构建复杂形状的能力,在组织工程支架制备领域表现出无可比拟的优势。目的:利用三维打印技术制备掺铁介孔硅酸钙/3-羟基丁酸-3-羟基己酸共聚酯复合支架,并进行表征及细胞相容性实验。方法:利用三维打印技术制备含铁摩尔百分比分别为0%,5%,10%,15%的掺铁介孔硅酸钙/3-羟基丁酸-3-羟基己酸共聚酯复合支架,分别记为0Fe-MCS/PHBHHx、5Fe-MCS/PHBHHx、10Fe-MCS/PHBHHx及15Fe-MCS/PHBHHx;扫描电镜观察支架浸泡于模拟体液前后的微观结构。将成骨细胞株MC3T3-E1分别接种于4组支架上,培养1,3,7 d,CCK-8法检测细胞增殖;培养7,14 d,检测细胞碱性磷酸酶活性。结果与结论:(1)与浸泡前相比,在模拟体液中浸泡3 d后,各组支架上出现了由矿化而形成的球形颗粒;(2)培养第1天,4组间细胞增殖无差异;培养第3天,15Fe-MCS/PHBHHx组细胞增殖显著高于其他3组(P<0.05);培养第7天,10Fe-MCS/PHBHHx组、15Fe-MCS/PHBHHx组细胞增殖明显高于0Fe-MCS/PHBHHx组(P<0.05),且15Fe-MCS/PHBHHx组高于10Fe-MCS/PHBHHx组(P<0.05);(3)培养第7天,4组细胞碱性磷酸酶活性无差异;培养第14天,5Fe-MCS/PHBHHx组、10Fe-MCS/PHBHHx组、15Fe-MCS/PHBHHx组细胞碱性磷酸酶活性明显高于0Fe-MCS/PHBHHx组(P<0.05),15Fe-MCS/PHBHHx组支架显著高于其他3组(P<0.05);(4)结果表明,掺铁介孔硅酸钙/3-羟基丁酸-3-羟基己酸共聚酯复合支架具有促进细胞增殖和成骨分化的能力。BACKGROUND： Three-dimensional （3D） printing technique has showed unparalleled advantages in the field of tissue engineering scaffold preparation because of its outstanding merits of convenience, efficiency, controllability and ability to construct complex shapes. OBJECTIVE： To fabricate Fe-containing mesoporous calcium-silicate （MCS）/poly （3-hydroxybutyrate-co-3- hydroxyhexanoate） （PHBHHx） composite scaffolds using the 3D printing technique and to test the characterization and cellular biocompatibility of the composite scaffolds. METHODS： Four groups of Fe-containing MCS/PHBHHx composite scaffolds were fabricated using 3D printing technique. The molar percentage of Fe in these four groups was 0%, 5%, 10%, 15%, respectively and they were marked as 0Fe-MCS/PHBHHx, 5Fe-MCS/PHBHHx, 10Fe-MCS/PHBHHx and 15Fe-MCS/PHBHHx. The scanning electron microscopy was used to observe the microstructure of the scaffolds after being soaked in the simulated body fluid. Osteoblast cell lines MC3T3-E1 were seeded on these four groups of scaffolds as well. Cell counting kit-8 method was adopted to test the cell proliferation at 1, 3, 7 days of culture. Intracellular alkaline phosphatase activity was tested at 7 and 14 days of culture. RESULTS AND CONCLUSION： （1） Compared with the scaffolds with no soaking process, spherical particles were formed on the scaffolds because of mineralization after soaking 3 days in the simulated body fluid. （2） At 1 day of culture, there was no difference in cell proliferation among the four groups, At 3 days of culture, the proliferation rate of the 15Fe-MCS/PHBHHx scaffold was remarkably higher than that of the rest three groups （P 〈 0.05）. At 7 days of culture, the proliferation rate was significantly higher in the 10Fe-MCS/PHBHH and 15Fe-MCS/PHBHHx scaffolds than the 0Fe-MCS/PHBHH scaffold （P 〈 0.05）, as well as significantly higher in the 15Fe-MCS/PHBHHx scaffold than the 10Fe-MCS/PHBHH scaffold （P 〈 0.05）. （3） At 7 days of culture, no difference in

关 键 词：生物相容性材料 细胞增殖 组织工程 生物材料 骨生物材料 三维打印 生物相容性 硅酸钙 3-羟基丁酸-3-羟基己酸共聚酯 MC3T3-E1细胞 支架 CCK-8 碱性磷酸酶 

分 类 号：R318[医药卫生—生物医学工程]