音猬因子修饰纳米晶胶原基骨复合骨髓间充质干细胞修复股骨缺损  

Effect of bone marrow mesenchymal stem cells combined with Shh modified nano-hydroxyapatite/collagen for femoral defect repair

作　　者：唐宇星[1] 赵庆[1] 杨中萌[1] 叶永恒[1] 胡仁安[1] 

机构地区：[1]中山大学附属第五医院骨一科,广东省珠海市519000

出　　处：《中国组织工程研究》2017年第14期2180-2185,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:音猬因子作为一种Hedgehog信号蛋白,具有促使骨组织发育、生长及重构等生理功能。目的:观察音猬因子修饰的纳米晶胶原基骨复合骨髓间质干细胞修复大鼠股骨缺损的效果。方法:将48只SD大鼠随机分为4组,均建立股骨缺损模型,造模成功后14 d,实验组于骨缺损处植入音猬因子修饰的纳米晶胶原基骨-骨髓间充质干细胞复合物,支架组于骨缺损处植入纳米晶胶原基骨,细胞支架组植入纳米晶胶原基骨-骨髓间充质干细胞复合物,空白对照组不植入任何材料。修复后3,6,9,12周,进行骨缺损区X射线检查、骨密度及骨组织病理切片观察。结果与结论:(1)X射线检查:实验组修复后不同时间点的Lane-Sandhu X射线评分、骨密度值均高于其余3组(P<0.05);(2)苏木精-伊红染色:修复后12周,支架组有少量骨组织形成,无骨髓形成;细胞支架组有少量骨组织形成,无骨髓形成,可见残留支架;实验组植入物完全吸收,成熟骨组织和髓腔形成,腔内有骨髓形成;(3)扫描电镜观察:修复后12周,支架组有不规则排列的骨纤维和大量骨陷下窝;细胞支架组可见大量成骨细胞,但骨纤维仍然不规则;实验组有大量排列规则的骨小梁,可见哈佛系统;(4)结果表明:音猬因子修饰的纳米晶胶原基骨复合骨髓间质干细胞可促进骨缺损的修复。BACKGROUND： SSh as a Hedgehog signal protein can promote bone development, growth and remodeling. OBJECTIVE： To investigate the effect of bone marrow mesenchymal stem cells （BMSCs） combined with Shh modified nano-hydroxyapatite/collagen （nHAC） in the repair of femoral defects in rats.METHODS： Forty-eight Sprague-Dawley rats were randomly divided into four groups, and the model of femoral defects was established in these rats. At 14 days after modeling, experimental group was implanted with the BMSCs/Shh modified nHAC, scaffold group was implanted with simple nHAC, cell scaffold group was implanted with BMSCs/nHAC, and blank control group was without any implantation. At 3, 6, 9, 12 weeks after repair, X-ray examination, bone density measurement and bone biopsy in bone defect area were performed. RESULTS AND CONCLUSION （1） X-ray examination： The Lane-Sandhu X-ray score and bone mineral density value in the experimental group at different time points after operation were significantly higher than those in the other three groups （P 〈 0.05）. （2） Hematoxylin-eosin staining： 12 weeks after repair, a small amount of bone tissues but no bone marrow formed in the scaffold group; a small amount of bone tissues with absence of bone marrow formed in the cell scaffold group, and the residual scaffold was visible; in the experimental group, the scaffold was completely absorbed, and mature bone and medullary cavity formed with presence of bone marrow. （3） Scanning electron microscope observation： 12 weeks after repair, irregular arrangement of bone fibers and a large number of bone fossae were observed in the scaffold group; the cell scaffold group showed a large number of osteoblasts, but bone fibers still arranged irregularly; in the presence of the Haversian system, a large number of regularly arranged bone trabeculae were detective in the experimental group. These results elucidate that the Shh modified nHAC/BMSCs complex can promote the repair of bone defects. Subject headings： Bone

关 键 词：骨移植 干细胞 股骨 组织工程 生物材料 骨生物材料 纳米晶胶原基骨 骨缺损模型 骨髓间充质干细胞 音猬因子 

分 类 号：R318[医药卫生—生物医学工程]