巨噬细胞极化影响前列腺癌细胞的促血管形成能力  

Macrophage polarization affects angiogenesis of prostate cancer cells

作　　者：徐璐[1,2] 胡银英[1,2,3] 余艳荣 袁铿[1,2,3] 王一帆[1,2,3] 刘燕玲 王志刚[1,2,3] 闵卫平[1,2,3] 

机构地区：[1]南昌大学免疫与生物治疗研究所 [2]江西省免疫与生物治疗重点实验室 [3]江西省医学科学院,南昌330006

出　　处：《现代免疫学》2017年第3期177-183,共7页Current Immunology

基　　金：国家重大研究计划(91229119);国家自然科学基金(81560465);江西省社会发展项目(20141BBG70062)

摘　　要：研究巨噬细胞极化对人前列腺癌PC-3细胞促血管形成的作用,并探讨其机制。本实验成功诱导正常男性外周血单核细胞为M0、M1、M2和TAM,发现它们在形态学上差异明显。ELISA检测各亚型巨噬细胞条件培养液(condition medium,CM)表明:TGF-β在TAM中显著高于其他亚型,IL-10高表达于M2及TAM,而IL-12在M1中高表达(P<0.05)。RT-PCR结果表明:IL-6、IL-23与CXCL9在M1中表达显著增高(P<0.05),以及miRNA let-7b、let-7c比TAM表达显著增高(P<0.01)。体外血管形成实验结果显示M2、TAM能增强PC-3细胞的促血管形成(P<0.01)。因此,TAM亚型巨噬细胞促进PC-3细胞的血管形成机制可能与miRNA let-7b、let-7c高表达以及上述细胞因子的差异性表达有关。This research aimed to investigate the effect of four subtypes of macrophage resulting from different polarization on angiogenesis of human prostate cancer PC-3cells,and to address the underlying mechanism.Normal male peripheral blood monocytes were induced to differentiate into M0,M1,M2 and TAM subtypes which were significantly different in morphology.The expression of TGF-βwas significantly increased in the CM of TAM,and IL-10 expressed highly in M2 and TAM,whereas IL-12 was significantly increased in M1（P〈0.05）as evidenced by ELISA.At the same time,the expressions of IL-6,IL-23 and CXCL9 at mRNA level in M1 were significantly increased as compared with M0,M2 and TAM as detected by RT-PCR（P〈0.05）.let-7b and let-7c in TAM were significantly up-regulated compared with the other three subtypes（P〈0.01）;M2 and TAM could promote the angiogenesis of PC-3 cells（P〈0.01）.Therefore,the mechanism by which macrophage of TAM subtype to promote angiogenesis of PC-3cells may be related to the expressions of miRNA let-7band let-7cthrough regulating the expression of the cytokines examined in this study.

关 键 词：巨噬细胞 PC-3细胞 血管生成 MIRNA 

分 类 号：R392.9[医药卫生—免疫学]