气管内滴入与腹膜腔注射脂多糖致肺组织炎症损伤的动态变化比较  

Intraperitoneal instillation versus intratracheal injection of lipopolysaccharide:differences in establishment of acute lung injury model

作　　者：袁伟锋[1] 李理[1] 徐虹[1] 胡玉洁[2] 黄文杰[1] 

机构地区：[1]广州军区广州总医院呼吸内科,广东广州510010 [2]解放军第458医院呼吸内科,广东广州510602

出　　处：《中国呼吸与危重监护杂志》2017年第3期274-279,共6页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：国家自然科学基金面上项目(81370173);国家自然科学基金青年科学基金(8120002;81200025);广东省医学科学技术研究基金(A2016305);广东省科技计划项目(2016ZC0213)

摘　　要：目的比较气管内滴入脂多糖与腹膜腔注射脂多糖两种不同方法复制的急性肺损伤小鼠模型的生理、病理特点及动态变化。方法 BALB/c小鼠麻醉后分别经气管内滴入脂多糖(5 mg/kg)和经腹膜腔内注射脂多糖(5 mg/kg)。在脂多糖处理后第1、2、6、12、18、24、48 h处死小鼠,通过检测支气管肺泡灌洗液(BALF)蛋白含量、肺湿/干重比值和肺组织病理半定量评分评价呼吸系统损伤程度,ELISA法检测血清与BALF上清中肿瘤坏死因子-α(TNF-α)浓度以了解全身与肺局部炎症反应程度。结果两组脂多糖处理小鼠的BALF中蛋白含量、肺湿/干重比值、血清/BALF中TNF-α浓度均增高。腹膜腔注射脂多糖小鼠肺湿/干重比值较气管滴入脂多糖小鼠高,其余上述指标在两组间无显著差异。两组小鼠在脂多糖处理后肺组织炎症细胞浸润、肺泡间隔增宽、肺组织病理半定量评分均明显增高,以气管内滴入组病理损伤较显著。结论气管内滴入脂多糖与腹腔注射脂多糖均可引起肺局部炎症与组织损伤,两种模型病理损伤程度差异较大,自身好转趋势不同,应根据实验目的选择合适的模型复制方法。ObjectiveTo compare two different ways to establish mouse model with acute lung injury （ALI） via intratracheal instillation or intraperitoneal injection of lipopolysaccharide （LPS）. MethodsBALB/c mice received intraperitoneal/intratracheal administration of LPS or sham operation. Wet/dry lung weight ratio, protein concentration in bronchoalveolar lavage fluid （BALF）, and lung tissue histology were examined at 0, 1, 2, 6, 12, 18, 24, 48 h after LPS administration. Tumor necrosis factor-α （TNF-α） in BALF and serum was assayed with ELISA method. ResultsLPS treatment significantly increased wet/dry lung weight ratio, BALF protein concentration and TNF-α concentration in serum and BALF. Lung tissue was damaged after LPS challenge. The mice received LPS intraperitoneal injection got a more significant lung edema than those received LPS intratracheal instillation. Inversely, LPS intratracheal instillation induced more severed microstructure destruction. ConclusionsALI animal model by LPS intratracheal instillation or intraperitoneal injection induces inflammation and tissue damage in lung. However, the degree of tissue damage or self-healing induced by two methods is different. Therefore the decision of which way to establish ALI model will depend on the study purpose.

关 键 词：急性肺损伤 动物模型 气管滴入 腹膜腔注射 脂多糖 

分 类 号：R563.8[医药卫生—呼吸系统]