河北省石家庄市手足口病流行中健康人肠道病毒带毒情况及病原分析  

Investigation of the Prevalence of Enterovirus Carriers among Healthy Children and Pathogenic Analysis during HFMD Outbreaks in Shijiazhuang City,Hebei Province,China

作　　者：田会方[1] 张勇[2] 李秀娟[1] 徐保红[1] 

机构地区：[1]河北省石家庄市疾病预防控制中心,石家庄050011 [2]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《病毒学报》2017年第3期395-403,共9页Chinese Journal of Virology

摘　　要：为了解河北省石家庄市健康儿童中肠道病毒带毒情况及血清型构成,对2010~2012年石家庄市的3个县区1 223例0~6岁的健康儿童及406名监护人(健康成人)采集咽拭子和粪便标本进行核酸检测鉴定肠道病毒血清型,并进行分子流行病学分析,为肠道病毒感染相关疾病的防控和治疗提供基础资料。1 223名健康儿童的标本检测结果中,肠道病毒(Enterovirus,EV)阳性的283例,阳性率为23.14%(283/1 223)。其中,以非肠道病毒71型非柯萨奇病毒A组16型的EV(Other-Enterovirus,other-EVs)阳性为主,占EV阳性的62%(175/283),而引起手足口病常见的EV病原体肠道病毒71型(Enterovirus A71,EV71)和柯萨奇病毒A组16型(Coxsackievirus A16,CVA16)阳性率较低,分别占19%(55/283)和18%(51/283);将结果为other-EVs阳性的175名健康儿童的标本全部进行病毒分离鉴定,共分离到25株病毒,分离率为14.29%(25/175),其中柯萨奇病毒B组5型(Coxsackievirus B5,CVB5)分离率最高,为40%(10/25)、柯萨奇病毒B组13型(Coxsackievirus B3,CVB3)分离率为24%(6/25)、埃可病毒21型(Enteric cytopathic human orphan virus 21,ECHO21)分离率为16%(4/25)、其他EV株分离率为20%(5/25)。提示,应加强对CVB3和CVB5等other-EVs的监测。To ascertain the prevalence of enterovirus carriers among healthy children and serotype distribution,pharyngeal swabs and fecal samples from 1,223 healthy children （age 0-6 years） and 406 guardians were collected from three counties in Shijiazhuang City, Hebei Province, China. We undertook nucleic-acid testing, identification of enterovirus（EV） serotype, and analyses of the molecular epidemiology of these specimens. In this way, we wished to provide basic data to aid the prevention and treatment of viral disease in the intestine. Of the 1,223 samples collected, EV positivity was noted in 283 cases, （23.14%）. Among these 283 samples, positivity for EV71, CVA16 and other-EVs was noted in 55, 51 and 175. Results for the other-EVs positive specimens in 175 healthy children showed separation into 25 enterovirus strains （virus separation rate 14.29%）. Separation for CVB5 was seen in 10 samples （40%）, that for CVB3 in 6 （24%）, that for ECHO21 4 （16%）, and that for other-EVs strains 5 （20%）. Our data suggest that monitoring of other-EVs such as CVB3 and CVB5 should be strengthened.

关 键 词：手足口病(HFMD) 健康儿童 肠道病毒 核酸检测 带毒率 

分 类 号：R373.2[医药卫生—病原生物学]