虾夷扇贝苗种培育水中可培养细菌群落分析  

Characterization of culturable bacterial diversity in the water of Yesso scallop Patinopecten yessoensisis larva rearing tanks

作　　者：孙雪莹[1] 刘继晨[1] 李明 赵学伟 梁峻 孙丕海[3] 马悦欣[1] SUN Xue-ying LIU Ji-chen LI Ming ZHAO Xue-wei LIANG Jun SUN Pi-hai MA Yue-xin(Key Laboratory of Mariculture and Stock Enhancement in North China＇s Sea, Ministry of Agriculture, Dalian Ocean Lniversity, Dalian 116023, Chi-na Zhangzidao Island Group Company Limited, Dalian 116001, China Seafood Seedling Breeding Base, Dalian Ocean Lniversity, Dalian 116023, China)

机构地区：[1]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023 [2]獐子岛集团股份有限公司,辽宁大连116001 [3]大连海洋大学海珍品苗种培育基地,辽宁大连116023

出　　处：《大连海洋大学学报》2017年第3期323-328,共6页Journal of Dalian Ocean University

基　　金：国家科技支撑计划项目(2013BAD23B01);獐子岛集团资助项目(99801214)

摘　　要：为研究虾夷扇贝Patinopecten yessoensisis苗种培育过程中幼体发育5个时期苗种培育水中(受精卵期水样W1,担轮幼虫期水样W2,D型幼虫期水样W3,壳顶幼虫期水样W4,稚贝期水样W5)可培养细菌的群落组成,采用传统培养方法,从每个水样分离细菌30株,通过16S rRNA基因测序分析鉴定细菌。结果表明:分离的150株细菌可归为2门、3纲、6目、9科、10属、22种;在门水平,W1~W5的优势门均为变形菌门,其次为厚壁菌门;在属水平,假交替单胞菌属为所有水样第一优势属,此外,W1的优势属依次为交替单胞菌属、弧菌属和海杆菌属,W2的优势属依次为弧菌属、交替单胞菌属和Cobetia,W3的优势属依次为弧菌属、交替单胞菌属和芽孢杆菌属,W4的优势属依次为交替单胞菌属、芽孢杆菌属和弧菌属,W5的优势属依次为弧菌属、Pseudophaeobacter、芽孢杆菌属和赤杆菌属。研究表明,扇贝幼体不同发育时期苗种培育水中所含可培养细菌种类比较丰富,细菌群落结构存在差异。This study was aimed to examine the culturable bacterial community in the water of larva rearing tanks during Yesso scallop Patinopecten yessoensisis larval development stages （fertilized egg W1, trochophora W2, D- shaped larvae W3 , umbo larvae W4, and juvenile scallop W5 ）. Thirty strains were isolated from each water sample by Zobell 2216E , and 150 bacterial strains were identified by polymerase chain reaction and partial 16S rRNA gene sequencing. Results showed that all isolates were found to be in 2 phyla, 3 classes, 6 orders, 9 families, 10 genera and 22 species. At phylum level, Proteobacteria was the most dominant phylum in 5 water samples. At genus lev-el ,the first dominant genus in all samples was Pseudoalteromonas , followed by Alteromonas , Vibrio and Marinobact- er at W1 stage, Vibrio, Alteromonas and Cobetia at W2 stage, Vibrio, Alteromonas and Bacillus at W3 stage, Altero-monas , Bacillus and Vibrio at W4 stage, and Vibrio, Bacillus, Pseudophaeobacter and Erythrobacter at W5 stage. The findings show that there are abundant bacterial species and different community structure in the water of larva rearing tanks across different larval development stages.

关 键 词：虾夷扇贝 育苗培育水 16S RRNA基因 细菌群落 

分 类 号：S917.1[农业科学—水产科学]