大麻二酚抑制小鼠脊髓损伤后炎症反应及促进功能恢复的研究  

Cannabidiol Can Inhibit Inflammation in a Mouse Model of Spinal Cord Injury

作　　者：李萃萃[1] 张曼辉 夏涵[3] 戴宜武[1] 

机构地区：[1]南方医科大学,广东广州510515 [2]陆军总医院附属八一脑科医院超声科,北京100010 [3]北京中医药大学东方医院麻醉科,北京100078

出　　处：《河北医学》2017年第5期767-770,共4页Hebei Medicine

基　　金：国家自然科学基金;(编号:81271391)

摘　　要：目的:探讨大麻二酚抑制小鼠急性脊髓损伤后炎症反应及其机制。方法:将C57/BL6小鼠随机分为3组(假手术组、对照组和用药组),每组8只,共24只。用药组和对照组小鼠用钳夹法夹断小鼠脊髓,假手术组不夹断脊髓,术后每日为小鼠挤压膀胱,帮助排尿。术后1h、12h、24h、36h、48h、60h给药,依据小鼠体重,对照组和假手术组腹腔注射安慰剂(0.25mL/10g,1%吐温80+生理盐水),用药组予以腹腔注射30mg/kg大麻二酚(1.2mg/mL,溶剂为1%吐温80+生理盐水)。术后72h评价小鼠脊髓神经功能,行BMS(Basso mouse scale,BMS)评分,并通过ELISA检测组织中高迁移率族蛋白1(HMGB1)和肿瘤坏死因子-α(TNF-α)含量。结果:BMS(Basso mouse scale,BMS)评分,假手术组>用药组>对照组(P<0.05);脊髓组织中HMGB1和TNF-α含量均为假手术组<用药组<对照组(P<0.05)。结论:大麻二酚能通过抑制脊髓损伤小鼠模型HMGB1表达来抑制小鼠炎症反应,从而对脊髓损伤小鼠脊髓神经功能保护。Objective： To investigate the inhibition of inflammation by cannabidiol in a mouse model of spinal cord injury and study its mechanism. Methords： 24 male C57B1/6 mice were randomized into 3 groups： sham-operation group, control group and medication group. We established an spinal cord injury model by clip method, the mice in sham-operate group and control group were administered with a mixture of 1% of tween 80 and sterile saline （0.25mL/10g） and medication group were treated with cannabidiol （30mg/kg ; was dissolved in a mixture of 1% of Tween 80 and sterile saline （1.2mg/mL） ） by intraperitioneal injection at 1h,12h,24h,36h,48h,60h after operation.72h after operation , BMS test was used to evaluate the neurologi- cal score, the expression levels of HMGB1 and TNF-α in spinal cord tissue were assayed by ELISA. Rsults： The BMS test showed that medication group had a higher score than the control group（P〈0.05）. The expression levels of HMGB1 and TNF-α in medication group were significant reduced as comparison with control group P〈0.05） , but significant increased than the sham-operation group（P〈0.05）. Conclusion： Cannabidiol can inhibit inflammation in a mouse model of spinal cord injury via inhibiting the expression of HMGB1.

关 键 词：大麻二酚 脊髓损伤 炎症 运动功能 

分 类 号：R651.2[医药卫生—外科学]