甜菜褐斑病抗性基因的克隆  

Molecular Cloning of Resistant Genes Related to Sugarbeet Cercospora beticola

作　　者：刘大丽[1] 马龙彪[1] 纪岩[2] 张革艳[2] 刘丽萍[2] 鲁振强[2] 

机构地区：[1]黑龙江省高等学校甜菜遗传育种重点实验室/黑龙江大学农作物研究院,哈尔滨150080 [2]黑龙江省高等学校生物化学与分子生物学重点实验室/黑龙江大学生命科学学院,哈尔滨150080

出　　处：《中国糖料》2017年第4期5-7,共3页Sugar Crops of China

基　　金：黑龙江省教育厅资助项目(12541608)

摘　　要：为了获得抗病的甜菜遗传材料,通过RT-PCR技术,分别对两类真菌抗性基因At Chitinase1(Gen Bank No.NM_100117)和At Glucanase2(Gen Bank No.NM_115586)的功能域进行了克隆;将所得的片段连接到载体pMD 18-T并鉴定。结果表明,At Chitinate1基因的核苷酸长度为819 bp,编码272个氨基酸,At Glucanase2基因的核苷酸长度为1020 bp,编码339个氨基酸,确认获得两个抗病基因功能片段。To improve the resistance of sugarbeet to Cercospora beticola Sacc., target genes AtChitinasel （GenBank No.NM_100117） and AtGlucanase2 （GenBank No. NM_115586） were selected, and the CDS of two genes were cloned respectively by RT-PCR. Two CDS were constructed into pMD 18-T Vector respectively, and identified with enzymes digested and sequenced. AtChitinasel and AtGlucanase2 were 819 bp and 1020 bp, deduced to encode 272 and 339 amino acids respectively, and identified as predicted.

关 键 词：甜菜 褐斑病 几丁质酶 葡聚糖酶 基因克隆 

分 类 号：S566.3[农业科学—作物学] Q78[生物学—分子生物学]