苹果赤霉素信号转导因子MdGAMYB的克隆和表达分析  

Cloning and Expression Analysis of the Gibberellin Signal Transduction Factor MdGAMYB in Malus

作　　者：樊胜[1] 雷超[1] 辛明志[1] 邵红霞[1] Muhammad Mobeen Tahir 张东[1] 韩明玉[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《园艺学报》2017年第5期817-827,共11页Acta Horticulturae Sinica

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-28);国家自然科学基金项目(31672101);陕西省科技统筹项目(2015NY114;2016KTZDNY01-10);中央高校基本科研业务费(2452015291);国家苹果改良中心杨凌分中心项目;陕西省果业发展协同中心项目

摘　　要：以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子MdGAMYB,对其进行生物信息学和表达分析。结果表明,MdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明MdGAMYB编码的蛋白存在多个糖基化位点和磷酸化位点;序列分析表明,Md GAYMB和其他物种的GAMYB蛋白有很高的相似性,均含有保守的R2R3 DNA结合域和GAMYB家族所特有的Box1,Box2和Box3保守区域;系统进化分析表明,Md GAYMB与梨、梅花、草莓、枣和葡萄等的GAMYB蛋白具有较高的同源性。实时荧光定量PCR分析表明,Md GAYMB具有组织表达特异性,在叶片、花和芽中的表达量较高。外源GA3处理抑制了花芽孕育和翌年成花,抑制MdGAMYB的表达。在易成花品种‘烟富6号’中的表达量高于难成花品种‘长富2号’。A cDNA encoding MdGAMYB was cloned from the terminal buds of‘Nagafu 2’apple. We further performed related research and analysis on its bioinformatics and expression patterns. Sequences analysis indicated that the opening reading frame of MdGAMYB was 1 656 bp and it encoded 551 amino acids. The molecular weight of MdGAMYB protein is 59.741 kD. Bioinformation showed that MdGAMYB protein had many glycosylation and phosphorylation sites. Amino acid sequence alignment showed that MdGAMYB was similar to other GAMYB proteins. They all shared a R2R3 DNA binding domain and typical conserved domains including Box1,Box2 and Box3. Phylogenetic analysis indicated that MdGAMYB protein was highly homologous to other GAMYB proteins,such as pear,plum,jujube,strawberry and grape. Real time PCR analysis showed that MdGAMYB was highly expressed in leaves,buds and flowers,exhibiting a tissue-specific expressed. Additionally,results showed that the MdGAMYB transcripts,flower induction and flowering rates were all inhibited by exogenous GA3 treatment. We found that the expression of MdGAMYB was higher in‘Yanfu 6’than‘Nagafu 2’during flower induction.

关 键 词：苹果 MdGAMYB 基因克隆 基因表达 成花诱导 

分 类 号：S661.1[农业科学—果树学]