miR-23及孕激素受体蛋白在子宫肌瘤组织中表达及临床意义研究  

The study on the expression of miR-23、pr receptor protein in uterus leiomyoma and its clinical significance

作　　者：史凡黎 宋燕[1,2] 王敏[1] 刘易斯[1] 史聪[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]济南市妇幼保健院,山东济南250001

出　　处：《中国实用妇科与产科杂志》2017年第6期655-658,共4页Chinese Journal of Practical Gynecology and Obstetrics

摘　　要：目的探讨miR-23和孕激素受体(PR)蛋白在子宫肌瘤组织中的表达及临床意义。方法分别用realtimePCR法及western blot法对2013年12月至2014年6月中国医科大学附属盛京医院30例子宫肌瘤组织、30例相应肌瘤包膜外子宫肌层组织及10例因子宫脱垂行子宫切除术的子宫肌层组织中的miR-23和PR蛋白进行检测,并用SPSS17.0统计分析软件对检测结果进行统计及相关性分析。结果 miR-23在相应包膜外子宫肌层组及正常子宫肌层组表达显著高于子宫肌瘤组织(P<0.001)。孕激素受体A(PRA)、孕激素受体B(PRB)蛋白在相应包膜外子宫肌层组及正常子宫肌层组表达显著低于子宫肌瘤组织(P<0.001)。比较相应包膜外子宫肌层组与正常子宫肌层组中PRA、PRB蛋白的相对含量,PRA相对含量差异无统计学意义(P=0.198),PRB相对含量差异有统计学意义(P=0.035)。在子宫肌瘤组织中,PRA、PRB蛋白表达差异无统计学意义(P=0.389)。miR-23与PRA蛋白表达之间存在负相关关系(r=-0.754),与PRB蛋白相对含量之间无相关关系(r=-0.394)。结论 miR-23与PR共同促进子宫肌瘤的发生与发展。PR为miR-23的靶基因之一,miR-23可能通过转录水平导致PRA蛋白增多。Objective To analyze the expressions of miR-23 and PR protein in uterine leiomyoma in order to find their clinical significance. Methods The reahime-PCR and Western blot methods were used to detect the expressions of miR-23 and PR protein.Results The expression of miR-23 in extracapsular myometrium and normal myometrium is higher than which in uterine leiomyoma tissue, and the differences both have significance （P〈O.O01）. PRA and PRB pro- tein are highly expressed in uterine leiomyoma, the low expression in the groups of normal myometrium and correspond- ing extracapsular myometrium, the differences both have significance （P〈0.001）.In corresponding extracapsular myome- trium and normal myometrium, the difference of PRA protein content has no significance （P=0.198） , the difference of PRB protein content has significance （P=0.O35）.In uterine leiomyoma, the difference of the relative content of PRA and PRB protein has no significance （P=0.389）.miR-23 and PRA protein are negative correlated in uterine leiomyoma tissue （r= -0.754）, miR-23 and PRB protein have no correlation（r=-0.394）.Conclusion miR-23 and PR have relationship with the development of uterine leiomyoma. PR is one of the target genes of miR- 23, which can lead to an increase of PRA protein in the level of transcription.

关 键 词：子宫肌瘤 miR-23 孕激素受体A 孕激素受体B 

分 类 号：R711.74[医药卫生—妇产科学]