大丽轮枝菌侵染陆地棉早期的转录组分析  

Transcriptome Analysis of Verticillium dahliae in Upland Cotton at Early Infection Stage by RNA-seq

作　　者：张志东[1] 段兴鹏[1] 周玉梅[1] 李建福[1] 韩松[1] 高正银 赵佳薇[1] 左开井[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《棉花学报》2017年第3期253-260,共8页Cotton Science

基　　金：国家科技重大专项--转基因生物新品种培育(2016ZX08005003-003)

摘　　要：【目的】旨在深入挖掘大丽轮枝菌致病相关基因。【方法】运用转录组测序技术比较分析了强致病力大丽轮枝菌Vd991在侵染陆地棉品种Coker 312早期阶段的基因表达情况。【结果】对Vd991侵染前后的转录组数据进行差异表达分析,共筛选到979个差异表达基因(Differentially expressed genes,DEGs),其中376个上调,603个下调。通过基因注释和功能分类发现,这些DEGs涉及细胞增殖与生长、产孢、碳水化合物结合、蛋白激酶活性调节、裂解酶活性、水解酶活性等功能。对上调DEGs进行基因本体(Gene ontology,GO)功能富集分析,发现共有277个GO词条达到显著富集水平(P<0.05),涉及生长速率正向调控、核苷酸合成、解旋酶活性等功能;京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路分析表明,上调DEGs参与53个代谢通路,包括嘧啶代谢、嘌呤代谢等。【结论】在早期侵染过程中,大丽轮枝菌细胞增殖相关基因表现活跃。上述分析为进一步揭示大丽轮枝菌的致病机理提供了有价值的参考。[Objective] The aim of this study is to mine pathogenesis related genes of Verticillium dahliae. [Method] We explored the transcriptome of highly toxic V. dahliae strain Vd991 at the early stage of infection in Coker 312, an upland cotton cultivar. [Result] Through differential expression analysis, a total of 979 differentially expressed genes （DEGs）were identified after infec- tion, of which 376 were up-regulated and 603 were down-regulated. The result of gene annotation and classification showed that these DEGs are involved in broad biological processes, molecular function and cellular component, including reproductive pro- cess, growth, sporulation, carbohydrate binding, kinase regulator activity, lyase activity and hydrolase activity. Enrichment anal- ysis of the up-regulated DEGs were performed, 277 gene ontology terms were enriched, including positive regulation of growth rate, purine nucleotide biosynthetic process, pyrimidine nucleobase biosynthetic process, helicase activity. A total of 53 Kyoto Encyclopedia of Genes and Genomes pathways were identified in the up-regulated DEGs, including purine metabolism, pyrimi- dine metabolism. [Conclusion] These results indicate that genes related to cell proliferation actively expressed at the early stage of infection and provide valuable reference for exploring the pathogenic mechanism of V. dahliae.

关 键 词：大丽轮枝菌 转录组分析 细胞增殖 致病机理 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]