缺铁性贫血合并心衰豚鼠乳头肌电生理的变化  

Electrophysiological changes of Papillary Muscles in Guinea Pigs with iron deficiency anemia and heart failure

作　　者：范凌[1] 陈立锋[2] 范静 

机构地区：[1]河北北方学院附属第一医院血液科 [2]河北北方学院基础医学院生理教研室 [3]河北张家口市第六医院妇产科,河北张家口075000

出　　处：《海南医学院学报》2017年第8期1014-1015,1019,共3页Journal of Hainan Medical University

基　　金：张家口市2013年度科学技术研究与发展计划项目(1321078D)~~

摘　　要：目的:探讨缺铁性贫血合并心衰豚鼠左心室乳头肌动作电位的变化。方法:制备缺铁性贫血合并心衰豚鼠模型20例为实验组,正常豚鼠10例为对照组。取血测定血红蛋白含量、红细胞数和全血铁指标,利用RM6240生物信号采集系统检测两组动物心功能和血流动力学的改变情况,确定造模是否成功;采用细胞内微电极技术测定模型组和对照组乳头肌动作电位,测定静息电位(RP)、超射值(OS)、动作电位幅值(APA)、0相最大除极速率(Vmax)、复极20%、50%和90%时间(APD_(20)、APD_(50)and APD_(90))及复极化平均速度,比较模型组和对照组之间有无统计学差异。结果:模型组存活的14例全部造模成功,模型组与对照组相比,APD_(50)、APD_(90)延长(P<0.01),复极化平均速度减慢(P<0.01)。结论:缺铁性贫血合并心衰豚鼠乳头肌动作电位复极化时程延长,平均复极化速度减慢。Objective： To investigate the changes of left ventricular papillary muscle action potentials in guinea pigs with iron deficiency anemia and heart failure. Methods： 20 cases of iron deficiency anemia with heart failure were treated with experimental group and 10 normal guinea pigs as control group. Blood samples were collected to determine hemoglobin content, red blood cell number and whole blood iron index, and the changes of cardiac function and hemodynamics were detected by 6,240 biological signal collection system to determine whether the model was successful or not, Intracellular microelectrode technique was used to determine the action potentials of the papillary muscles in the model group and the control group, the potential am- plitudes （APA）, overshoot values （APA）, maximum depolarization rate （Vmax）, 20 % of repolarization, 50 % and 90 % of repolarization （APD 20, APD 50 and APD 90） and the average velocity of repolarization were measured. Compare statistical difference between the model group and the control group. Results： 14 cases of model group survived completely, compared with control group, APD so and APD 90 prolonged （P〈0.01）, and the average velocity decreased （P〈0.01）. Conclusions： the action potential repolarization duration in the guinea pig papillary muscle of iron deficiency anemia with heart failure is prolonged, and the average repolarization velocity is slow.

关 键 词：缺铁性贫血合并心衰 豚鼠模型 乳头肌 动作电位 

分 类 号：R556.2[医药卫生—血液循环系统疾病]