邱氏穴对肾绞痛大鼠模型肾组织PGI2、TXA2和5-HT表达的影响  

Effect of Qiu's point on expressions of PGI2, TXA2 and 5-HT in rat model of renal colic

作　　者：王峻[1] 肖扬[1] 梁小兴 刘鸿[1] 王志刚[1] 孟磊[1] 姚睿智[1] 陈铭[1] 邱云桥[1] 

机构地区：[1]广州中医药大学第一附属医院泌尿外科,广东广州510405

出　　处：《中国现代医学杂志》2017年第10期35-39,共5页China Journal of Modern Medicine

基　　金：广州中医药大学第一附属医院创新强院工程项目(2015JY04)

摘　　要：目的观察邱氏穴对肾绞痛大鼠模型中肾组织中前列环素(PGI2)、血栓素A2(TXA2)以及5羟色胺(5-HT)表达的影响。方法将SD大鼠分为3组:正常组、模型组和邱氏穴组。模型组与邱氏穴组采用1%乙二醇和1%氯化铵水溶液作为诱石剂自由饮水10 d建立草酸钙结石致大鼠肾绞痛模型,邱氏穴组采用龙虎交战复合手法针刺大鼠邱氏穴,通过观察大鼠行为学和肾组织病理切片变化,检测肾组织中PGI2、TXA2和5-HT表达来判定邱氏穴对肾绞痛的影响作用。结果正常组与邱氏穴组中痛阈值测定及自发性疼痛评分均较模型组降低(P<0.05),模型组中PGI2、TXA2和5-HT表达均较正常组及邱氏穴组升高(P<0.05),正常组与邱氏穴组PGI2、TXA2和5-HT表达比较差异无统计学意义(P>0.05),邱氏穴组中PGI2/TXA2比值与正常组比较差异有统计学意义(P<0.05),与模型组中差异无统计学意义(P>0.05)。结论邱氏穴复合针刺手法对肾绞痛大鼠模型具有镇痛作用,推测其作用机制为通过抑制炎症介质PGI2、TXA2及神经递质5-HT合成,减轻5-HT致痛作用。Objective To observe the effect of Qiu＇s point on the expressions of PGI2, TXA2 and 5-HT in the rats with renal colic. Methods The SD rats were randomly divided into three groups： normal group, model group and Qiu＇s point group. In the model and Qiu＇s point groups, 1% ethylene glycol and 1% ammonium chloride were used as lure stone agents to establish the renal colic model induced by calcium oxalate stone in 10 days. The rats in the Qiu＇s point group were given acupuncture on Qiu＇s point with dragon-tiger fighting needling method. Then the behavior and pathological changes of kidney tissues were observed in the three groups, and the expressions of TXA2, PGI2 and 5- HT in the renal tissues were detected. Results The pain threshold value, the spontaneous pain score and the expressions of TXA2, PGI2 and 5-HT of the normal and Qiu＇s point groups were significantly lower than those of the model group （P 〈 0.05）; however, there was no significant difference in the TXA2, PGI2 or 5-HT expression between the normal group and the Qiu＇s point group （P 〉 0.05）. The PGI2/TXA2 ratio was significantly different between the Qiu＇s point group and the normal group （P 〈 0.05）, but not significantly different between the Qiu＇s point group and the model group （P 〉 0.05）. Conclusions Dragon-tiger fighting needling method on Qiu＇s point can achieve analgesic effect in the treatment with renal colic rats, the mechanism is perhaps inhibiting the synthesis of inflammatory mediators TXA2 and PGI2 and neurotransmitter 5-HT, and relieving the pain induced by 5-HT.

关 键 词：邱氏穴 龙虎交战 肾绞痛 大鼠 作用机制 

分 类 号：R332[医药卫生—人体生理学] R691.4[医药卫生—基础医学]