HPLC同时测定糖痹康颗粒中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量  

Simultaneous determination of Baicalin,Wogonoside,Baicalein and Wogonin in Chinese herbal compound Tangbikang by HPLC

作　　者：王东超[1] 魏颖[1] 高佳琪[1] 孙文[1] 秦灵灵[1] 兰卫[2] 石浩霞 李博[1] 徐暾海[1] 刘铜华[1] 

机构地区：[1]北京中医药大学,北京100029 [2]新疆医科大学,乌鲁木齐830011

出　　处：《吉林中医药》2017年第5期513-517,共5页Jilin Journal of Chinese Medicine

基　　金：国家科技重大专项(2012ZX09102201-001)

摘　　要：目的建立同时测定糖痹康颗粒中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素含量的方法。方法采用高效液相色谱法。色谱柱:Thermo syncronis C18(250 mm×4.6 mm,5μm);流动相:0.1%甲酸乙腈(A)-0.1%甲酸水(B);梯度洗脱:0~3 5 m i n,2 0%(B)~5 5%(B);3 5~4 0 m i n,1 0 0%(B);流速:1 m L/min;柱温为40℃;检测波长为275 nm;进样量为20μL。结果黄芩苷检测浓度在5~500μg/m L范围内与峰面积积分值呈良好线性关系Y=6×107X+62726(r=0.9996,n=7);平均回收率为100.06%,RSD=1.08%(n=9);汉黄芩苷检测浓度在1~85μg/m L范围内与峰面积积分值呈良好线性关系Y=90214X-51875(r=0.9997,n=7);平均回收率为100.11%,RSD=0.41%(n=9);黄芩素检测浓度在0.4~25μg/m L范围内与峰面积积分值呈良好线性关系Y=158201X-10020(r=0.9997,n=7);平均回收率100.00%,RSD=0.73%(n=9);汉黄芩素检测浓度在0.1~10μg/m L范围内与峰面积积分值呈良好线性关系Y=69606X-29918(r=0.9991,n=7);平均回收率为100.07%,RSD=1.17%(n=9);结论该方法操作简单,结果可靠,可为糖痹康颗粒的质量控制提供参考。Objective To establish a method of simultaneous determination of Baicalin, Wogonoside, Baicalein, and Wgonin in Chinese herbal compound Tangbikang. Methods HPLC was performed on the column ofThermo syncronis C18（250 mm× 4.6 mm, 5μm） with mobile phase of Formic acid acetonitrile （A） - 0.1% and Formic acid water （B） with gradient elution 0-35 min, 20% （B）-55% （B）; 35 min-40 min, 100% （B）; The flow rate of 1 mL/min, column temperature was 40℃ ; Detection wavelength was 275 nm; The volume injection was 20 μL. Results The linear range was 5-500μg/mL for Baicalin and the peak area of integral value with good linearity, its regression eqration wasY=6 ×107X＋ 62726 （r= 0.9996, n = 7）; The average recovery was 100.06%, RSD = 1.08% （n = 9）; The linearrange was 1-85μg/mL for Wogonoside and the peak area of integral value with good linearity, its regression eqration was Y=90214X-51875 （r= 0.9997, n = 7）; The average recovery was 100.11%, RSD = 0.41% （n = 9）; The linear range was 0.4-25 μg/mL for Baicalein and the peak area of integral value with good linearity, its regression eqration was Y=158201X-10020 （r = 0.9997, n = 7; The average recovery was 100.00%, RSD=0.73% （n=9）; The linear range was 0.1-10 μg/mL for Wogonin and the peak area of integral value with good linearity, its regression eqration was Y=69606X-29918 （r= 0.9991, n = 7）; The average recovery was 100.07% , RSD=1.17% （n =9）. Conclusions The method is simple, reliable, which can be provided reference for the quality control of Chinese herbal compound Tangbikang granules.

关 键 词：糖痹康 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 

分 类 号：R255.4[医药卫生—中医内科学]