男性不育患者精液中血管内皮生长因子及其受体Flt-1的表达研究  

Expressions of vascular endothelial growth factor and its receptor Flt-1 in semen of male infertility

作　　者：焦瑞宝[1] 唐吉斌[1] 冯恒孝[1] 刘志宏 姚余有[3] 

机构地区：[1]安徽省铜陵市人民医院,铜陵244009 [2]铜陵市义安区血吸虫防治站 [3]安徽医科大学公共卫生学院

出　　处：《中国男科学杂志》2017年第1期23-26,共4页Chinese Journal of Andrology

基　　金：安徽省铜陵市卫计委科研项目(卫科研[2014]28号)基金资助

摘　　要：目的研究男性不育患者精液中血管内皮生长因子(VEGF)及其受体1(Flt-1)的表达情况,探讨两者在精子发生和成熟中的作用。方法在完成男性不育患者精液计算机辅助分析后,选择性收集120例患者精液标本1mL置于-80℃冰箱保存,VEGF和Flt-1均采用ELISA试剂盒检测。结果研究对象中少精子症组的VEGF为(154.16±151.46)ng/L,与对照组(253.42±60.02)ng/L比较,t=2.490,差异有显著性(P<0.05);经Pearson相关性分析,Flt-1与VEGF呈正相关(r=0.675,P<0.01)。结论男性不育患者精浆中VEGF含量降低可能引起精子发生减弱,VEGF及其受体Flt-1的表达改变可影响精子运动特征。Objective To explore the expression of vascular endothelial growth factor （VEGF） and its receptor Flt-1 in semen of male infertility and their roles in spermatogenesis and sperm maturation. Methods Semen analysis was performed using computer-aided detection system, and then 120 semen specimens of male infertility patients were collected selectively and preserved in -80℃ refrigerator. The expressions of VEGF and Flt-1 were detected by ELISA. Results The expression of VEGF in asthenozoospermia group was （154.16±151.46）ng/L, while the expression of which in control group was （253.42±60.02） ng/L, the difference was statistically significant （P〈0.05）. Correlation coefficient r of Flt 1 and VEGF was equal to 0.675, correlation analysis showed a significant difference （P〈0.01）. Conclusion Lower VEGF levels in male infertility might cause less Spermatogenesis, the expression of VEGF and its receptor Flt-1 could affect the characteristics of sperm motility.

关 键 词：不育 男性 血管内皮生长因子类 精子能动性 

分 类 号：R698.2[医药卫生—泌尿科学]