低能量激光击打精子尾部鉴别存活精子中应用  

Strike sperm tails with low energy laser to identify viable sperm

作　　者：叶明建[1] 朱卫中[1] 赵恩慧[1] 李金美[1] 胡晓薇[1] 

机构地区：[1]浙江省丽水市人民医院生殖中心,浙江丽水323100

出　　处：《中国男科学杂志》2017年第1期27-30,共4页Chinese Journal of Andrology

摘　　要：目的验证激光击打法鉴别存活精子的可行性。方法选用不同脉冲输出时间的激光击打精子尾部的不同位置,观察活精子和死精子的形态变化,评估激光击打效果,选择合适的脉冲输出时间,确定理想的击打部位。采用激光击打法和伊红-苯胺黑活体染色两种方法检测不同来源的精子样本,分析两种方法检出存活精子的结果相关性。结果采用脉冲输出时间100μs的激光击打精子尾部的3/4位置,可收到较好的击打效果。受低能量激光击打后有90%活精子出现形态变化,其中75%表现为明显变化,5%呈现明显的颈部折角,而死精子形态无任何变化。激光击打法的存活精子检出率与精子活体存活率呈高度正相关(r=0.9925),但前者明显低于后者(P<0.05)。结论低能量激光击打法是鉴别存活精子简便而有效的方法,但需要注意操作要领。Objective To investigate the feasibility of single laser shot method for identifying viable spermatozoa. Methods We gave the sperm a shot at the different site of the tail using laser with different pulse time, and then observed the morphology changes of sperm for determining suitable pulse time of laser and the right shooting position. We also detect the survival rate of sperms from different sources using laser and staining, then analyzed its correlation. Results Laser with pulse time of 100us and three quarters of the sperm tail had a better result. After giving a shot, 90% of live sperms exhibited changes in morphology. Among them, 75% had significant changes and 5% presented neck angle, but the dead sperm had no change in morphology. The survival rate of two methods above showed positive correlation（r =0.9925）, but the laser method was significantly lower in survival rate（P〈0.05）. Conclusion Low energy laser is a simple and effective method for identifying viable sperm, but you should pay attention to operation details.

关 键 词：生殖技术 辅助 精子注射 细胞质内 精子 激光 

分 类 号：R698.2[医药卫生—泌尿科学]