黄芪散对糖皮质激素性骨质疏松大鼠股骨和胫骨的影响  

Effects of Huangqi Powder on femur and tibia in rats with glucocorticoid-induced osteoporosis

作　　者：王芳[1] 陈珺[2] 曾煦欣[3] 张家豪[1] 梁丹丹[1] 李卫民[4] 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]广东药科大学生命科学与生物制药学院,广东广州510006 [3]佛山科学技术学院口腔医学院,广东佛山528000 [4]广州中医药大学中药学院,广东广州510006

出　　处：《中成药》2017年第6期1109-1114,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金面上资助项目(81473439);2017年广东省普通高校青年创新人才项目(2017)

摘　　要：目的探讨黄芪散(葛根、黄芪、桑白皮)对糖皮质激素骨质疏松大鼠股骨和胫骨的影响。方法采用1月龄Sprague Dawley(SD)大鼠30只,随机均分3组:正常组(生理盐水)、模型组(醋酸泼尼松)、黄芪散组(剂量选择临床成人剂量)。实验维持120 d。动物处死前进行钙黄绿素双荧光标记。测定血清骨吸收和骨形成指标,采用骨生物力学方法测量股骨结构力学和材料力学参数,采用骨组织形态计量学方法测量胫骨上段松质骨和中段皮质骨的静态、动态参数。结果黄芪散组不能明显改善糖皮质激素对血液和骨生物力学造成的影响,但可明显增加糖皮质激素模型大鼠胫骨上段的骨小梁面积和骨小梁数目、胫骨上段荧光标记周长百分数、胫骨中段骨内膜骨矿化沉积率。结论黄芪散可明显提高糖皮质激素性骨质疏松大鼠胫骨松质骨骨量,增强骨小梁结构。AIM To study the influence of Huangqi Powder（Puerariae lobatae Radix,Astragali Radix,Mori Cortex） on femur and tibia in rats with glucocorticoid-induced osteoporosis（GIOP）.METHODS Thirty one-month-old male SD rats were divided into three groups by randomized block design,which were control group（normal saline）,prednisone group（3.5 mg/kg prednisone acetate）,Huangqi Powder group（clinical adult dosage）.Experimental test was maintained for one hundred and twenty days.Double fluorochrome labeling with calcein was performed before necropsy;bone resorption and bone formation index were determined by using blood serum;the femur structural and material mechanics were analyzed by using bone biomechanics method.The cancellous bone of proximal tibial and the cortical bone of tibial shaft static parameter and dynamic parameter were analyzed by bone histomorphometry method.RESULTS Huangqi Powder had no obvious improving effects on bone biomechanics and bone static parameter,but could increas the area,width and number of the proximal tibia bone trabecular.Mark perimeter percentage and mineralization rate of deposition could be increased.CONCLUSION Huangqi Powder can significantly increase proximal tibia cancellous bone mass and enhance bone trabecula structure of glucocorticoid-induced osteoporosis rats.

关 键 词：糖皮质激素性骨质疏松 黄芪散 骨生物力学 骨组织形态计量学 股骨 胫骨 

分 类 号：R285.5[医药卫生—中药学]