比较3种药对慢性哮喘大鼠的抗哮喘作用  

Comparison of three herbal proparations in airway remodeling in rats with chronic asthmatic

作　　者：高月娟[1] 孙琳林[1] 王景欣[1] 刘金丽[1] 佟雷[2] 

机构地区：[1]牡丹江医学院附属红旗医院,黑龙江牡丹江157011 [2]牡丹江医学院,黑龙江牡丹江157011

出　　处：《中成药》2017年第6期1119-1125,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学青年基金资助项目(81403316);牡丹江医学院科学技术研究项目(ZS201527);黑龙江省卫生和计划生育委员会科研课题(2016-369)

摘　　要：目的研究左归丸、右归丸和玉屏风散的抗哮喘作用及其作用机制差异。方法雄性Brown Norway大鼠随机分为5组,卵清白蛋白诱导慢性哮喘模型,给药组分别灌胃给予左归丸、右归丸、玉屏风散,正常组和模型组给予生理盐水,每日给药1次,连续给药14 d。测定大鼠Penh值,血清辅助性T淋巴细胞亚群Th1、Th2炎症细胞因子水平及皮质酮(CROT)和促肾上腺皮质激素(ACTH)蛋白水平。处死大鼠,取肺组织切片定量测定气道重塑病理变化。RT-PCR法测定下丘脑促肾上腺皮质激素释放激素(CRH)及肺组织TGF-β1、Smad3 mRNA表达水平。结果玉屏风散组、右归丸组Penh值显著低于左归丸组。玉屏风散组Th1因子显著高于左归丸组,Th2因子显著低于左归丸组。右归丸组仅白细胞介素IL-5显著低于左归丸组。玉屏风散组血清CROT、ACTH蛋白及下丘脑CRH mRNA显著高于左归丸组,右归丸组ACTH蛋白及CRH mRNA显著高于左归丸组。玉屏风散组肺组织转化生长因子TGF-β1、Smad3 mRNA表达水平与左归丸组无显著差异,右归丸组Smad3 mRNA表达水平显著低于模型组。玉屏风散和左归丸对气道重塑具有显著抑制作用。右归丸杯状细胞数显著低于左归丸组。结论左归丸、右归丸、玉屏风散均具有显著抗哮喘作用。玉屏风散对Th1/Th2平衡、下丘脑-垂体-肾上腺轴(HPA轴)及TGF-β1/Smad3通路的调节作用均较强;右归丸对TGF-β1/Smad3通路调节作用弱于左归丸,对HPA轴调节作用强于左归丸。AIM To research the antiasthmatic effects of Zuogui Pills,Yougui Pills and Yupingfeng Powder and their mechanism of action.METHODS Male Brown Norway rats were randomly divided into five groups.OVA was used to induce the model of chronic asthma.Five groups of asthmatic rats were given Zuogui Pills,Yougui Pills,Yupingfeng Powder by gavage once daily for 14 d,respectively.The normal and the model group were given normal saline.Penh value,serum Th1,Th2 inflammatory cytokines,and CROT and ACTH protein levels were determined.Pathological changes of airway remodeling were measured.RT-PCR method was used to measure the expression of CRH mRNA in hypothalamus,and TGF-β1 and Smad3 mRNA in lung tissues.RESULTS Penh values of Yupingfeng Powder group and Yougui Pills group were significantly lower than that of Zuogui Pills group.The Th1 inflammatory cytokine of Yupingfeng Powder group was significantly higher than that of Zuogui Pills group,while the Th2 cytokine was significantly lower than that of Zuogui Pills group.In Yougui Pills group,only IL-5 was significantly lower than that of Zuogui Pills group.Serum CROT,ACTH protein and CRH mRNA of Yupingfeng Powder group were significantly higher than those in Zuogui Pills group.No significant difference was found between Yupingfeng Powder group and Zuogui Pills group with regard to TGF-β1 and Smad3 mRNA.Smad3 mRNA of Yougui Pills group was significantly higher than that of the model group.Airway remodeling was significantly reduced by Yupingfeng Powder and Zuogui Pills.The number of goblet cells in Yougui Pills group was significantly lower than that in Zuogui Pills group.CONCLUSION All the three formulaes possess significant antiasthma effects.Regulation effects of Yupingfeng Powder on Th1/Th2 balance,HPA and TGF-β1/Smad3 pathway are strong.Compared with Zuogui Pills,Yougui Pills possesses weaker TGF-β1/Smad3 regulative effects and stronger HPA regulative effects.

关 键 词：左归丸 右归丸 玉屏风散 哮喘 机制 

分 类 号：R285.5[医药卫生—中药学]