杜鹃兰种子萌发适宜培养基的筛选  

Media Screening on Seeds Germination of Cremastra appendiculata

作　　者：王汪中 张明生[1] 吕享[1] 吴彦秋[1] 颜跃跃 叶睿华 

机构地区：[1]贵州大学生命科学学院,山地植物资源保护与种质创新省部共建教育部重点实验室,贵州贵阳550025

出　　处：《北方园艺》2017年第11期157-161,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(81360613,81660627);国家重点研发计划课题资助项目(2016YFC0502604);贵州省科技创新人才团队建设专项资金资助项目(黔科合平台人才[2016]5624);贵州省高层次创新型人才培养计划资助项目(黔科合人才(2015)4031号);贵州省教育厅创新群体重大研究资助项目(黔教合KY字[2016]023)

摘　　要：以人工授粉获得的杜鹃兰种子为试材,研究了不同基本培养基、不同有机添加物、蔗糖浓度和不同植物生长调节剂对杜鹃兰种子萌发的影响,筛选适于杜鹃兰种子萌发的培养基。结果表明:KC基本培养基上种子萌发较好,萌发率达17.38%;添加7.5%土豆泥对种子萌发较好,萌发率为15.36%;蔗糖浓度为1.0%时,种子有较高萌发率,为14.20%;添加0.5mg·L^(-1)KT或1.5mg·L^(-1) IBA时适于种子萌发,萌发率分别为36.19%、30.00%;正交实验得出适于杜鹃兰种子萌发的培养基为KC+0.5mg·L^(-1) KT(激动素)+1.3mg·L^(-1) IBA(吲哚乙酸)+1.0%蔗糖+7.5%土豆泥+0.5%活性炭,萌发率达32.87%,萌发时间约6周。Cremastra appendiculata seeds from artificial pollination were used as test materials to screen the suitable medium for seeds germination.The test contents involved the effects of the basic medium,organic additives,sucrose concentration,and growth regulators to seeds germination of C.appendiculata.The results showed that seeds which was in KC basic medium had higher germination rate（17.38%）.Adding 7.5% mashed potatoes into KC medium was beneficial to seeds germination,and the germination rate was 15.36% The seeds germination rate was higher（14.20%） when sucrose concentration was 1.0%.0.5 mg·L-1 KT or 1.5 mg·L-1 IBA was the suitable growth regulators seeds germination and the germinationrate was 36.19% and 30.00%,respectively.In conclusion,the best medium for seeds germination of C.appendiculata was KC＋0.5 mg·L-1 KT＋1.3 mg·L-1 IBA＋1.0% sucrose＋7.5% mashed potatoes＋0.5% AC,which seeds germination rate reached 32.87% and germination time was 6 weeks.

关 键 词：杜鹃兰 种子萌发 培养基 筛选 

分 类 号：S682.31[农业科学—观赏园艺]