柚子皮精油提取及其驱蚊效果研究  

Extraction and Mosquito Repellent Activity of Essential Oil from Citrus maxima(Burm.) Merr. cv. Shatian Yu peels

作　　者：杨愈丰 柳敦耀 向红英[1,3] 吕延成[1] 王菊芳[2] 

机构地区：[1]遵义医学院珠海校区生物化学与分子生物学教研室,珠海519041 [2]华南理工大学生物科学与工程学院,广州510640 [3]珠海市中药基础与应用研究重点实验室,珠海519041

出　　处：《天然产物研究与开发》2017年第5期867-872,共6页Natural Product Research and Development

基　　金：贵州省科技合作计划(黔科合LH字[2015]7547号);遵义医学院博士启动基金(F-712);珠海市重点实验室开放基金(ZYKL-2016K2)

摘　　要：本文通过响应面法研究了柚子皮精油的提取工艺,并初步考察了所提取精油的驱蚊效果。柚子皮精油提取采用微波辅助蒸馏法,在采用单因素实验考察蒸馏时间、料液比和NaCl质量分数对精油提取率的影响之后,通过响应面法进行提取工艺优化,以获得最佳提取方案;然后采用趋避法验证柚子皮精油的驱蚊活性。结果表明,最佳柚子皮精油提取方案如下:蒸馏时间100 min,料液比0.3∶2(g/m L),NaCl的质量分数1%,采用该方案柚子皮精油提取率达1.65%。通过初步驱蚊实验发现柚子皮精油具有较好的驱蚊活性,和市售六神花露水效果相当,驱蚊效应具有进一步深入研究的价值。The essential oil was extracted from Citrus maxima （Burm.） Merr. cv. Shatian Yu peels with distillation method. Response surface method was used to optimize the extraction process based on single factor experiments. Then the mosquito repellent activity of essential oil was tested by phobotaxis. The optimal extraction process were determined as follows： 30 g of pomelo peel was treated in 200 mL of buffer containing 1% of sodium chloride for 100 min,of which the extraction yield was up to 1.65%. The extracted essential oil has considerable mosquito repellent activity,which deserve to be researched further. The result of this study provided scientific basis for the application of C. maxima essential oil.

关 键 词：柚子皮精油 蒸馏法 响应面法 驱蚊活性 

分 类 号：R184.31[医药卫生—流行病学]