寿光鸡AANAT基因5′调控区的单核苷酸多态性及其与产蛋性状的关联分析  

SNPs in 5' Regulatory Region of AANAT Gene and the Association Analysis with Laying Traits in Shouguang Chickens (Gallus gallus)

作　　者：李秀秀[1] 王文文[1] 郝园丽 李显耀[1] 唐辉[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《农业生物技术学报》2017年第6期921-929,共9页Journal of Agricultural Biotechnology

基　　金：山东省现代农业产业技术体系项目(No.SDAIT-11-02)

摘　　要：褪黑激素(melatonin,Mel)分泌增加可阻碍家禽生殖器官的发育,降低家禽产蛋量。5-羟色胺-N-乙酰基转移酶(arylalkylamine N-acetyltransferase,AANAT)是Mel合成过程中的限速酶。本实验旨在研究AANAT基因5'调控区多态性及其与寿光鸡(Gallus domesticus'Shouguang')产蛋性能的关系,采用直接测序法检测了AANAT基因5'调控区的SNPs,并将检测到的SNPs与寿光鸡的产蛋性状进行关联分析。结果表明,AANAT基因5'控区共21个SNPs,其中10个低度多态、11个中度多态。3个中度多态位点对产蛋性能影响显著(P<0.05),其中-803位点能使开产日龄显著提前,并能使前期(20~25周龄)产蛋数显著增加;-561位点能使中期产蛋数显著增加;-522位点能使后期(46~57周龄)产蛋数显著增加;-561、-522位点能使产蛋总数显著增加。综上所述,AANAT基因5'调控区有多个SNPs对产蛋性状影响显著,可作为寿光鸡产蛋性状辅助选择的有潜在利用价值的分子遗传标记。The increase of melatonin （Mel） can obstruct the development of reproductive organs and reduce the egg production in poultry. The arylalkylamine N-acetyltransferase （AANAT） is the major rate-limiting enzyme for melatonin synthesis from serotonin. In order to investigate the polymorphism of the AANAT gene on laying traits, SNPs in 5＇ regulatory region of the gene were detected with the direct sequencing and then their correlations with the laying traits were analyzed in Shouguang chickens （Callus domesticus）. The results showed that 10 low polymorphism loci and 11 moderate polymorphism loci were found in the 5＇ regulatory region, respectively. Three SNPs with moderate polymorphisms had significant effects on laying traits （P〈 0.05）. The locus of-803 could significantly advance the age at first egg and increase the egg production during 20-25 weeks of age. The loci of-561 and-522 increased the egg production during 26-45 weeks of age and during 46-57 weeks of age, respectively, so as to significantly increased the total egg production as well. In summary, the 5＇ regulatory region of AANAT gene have many SNPs, which were significantly associated with laying traits and thus are potential molecular markers to improve laying traits in Shouguang chickens.

关 键 词：寿光鸡 5-羟色胺-N-乙酰基转移酶基因(AANAT) 产蛋性状 SNPS 关联分析 

分 类 号：S831.2[农业科学—畜牧学]