微波硫灯、热辐射灯、日光灯和LED灯对雄性抑郁大鼠生殖功能的影响  

Effects of sulfur lamp,heat radiation lamp,fluorescent lamp and LED lamp lighting on reproductive system in depressive male rats

作　　者：竹梅[1] 侯倩伶 狄贯腾 冯敏[1] 王莎莉[1] 

机构地区：[1]重庆医科大学神经科学研究中心,重庆400016

出　　处：《基础医学与临床》2017年第6期821-827,共7页Basic and Clinical Medicine

基　　金：重庆市渝中区科委基础与前沿科技项目[渝中科(2015)16号文]

摘　　要：目的探讨微波硫灯、热辐射灯、日光灯和LED灯对雄性抑郁大鼠生殖系统的影响。方法采用慢性、温和、不可预见性刺激(CUMS)方法建立抑郁大鼠模型。对模型大鼠分别给予微波硫灯、热辐射灯、日光灯和LED灯连续光照45 d后,摘取大鼠生殖器官测定其脏器系数比;ELISA检测血清中睾酮(T)、雌二醇(E2)、孕酮(PROG)及催乳素(PRL)含量;HE染色检测睾丸组织形态学变化,Western blot检测睾丸组织中褪黑素受体(Mel 1a)、胆固醇侧链裂解酶(P450scc)以及3β-羟类固醇脱氢酶(3β-HSD)的蛋白表达。结果与对照组相比,抑郁模型大鼠体质量增长缓慢,睾丸、附睾及精囊腺脏器系数均明显降低(P<0.05),血清中T、E2、PRL及PROG水平显著下降(P<0.05),睾丸间质充血严重,生精小管萎缩,细胞排列分散,Mel 1a、3β-HSD和P450scc表达下调(P<0.05)。微波硫灯照射可以上调T、E2、PRL和PROG的水平以及Mel 1a、3β-HSD和P450scc的表达(P<0.05),减轻睾丸组织病理学改变。结论微波硫灯照射可以改善雄性抑郁大鼠的睾丸结构及功能。Objective To investigate the effects of different lighting（ sulfur lamp, heat radiation lamp, fluorescent lamp and LED lamp） on reproductive system in depressive male rats. Methods CUMS depressive model of rats was established through the stimulations of chronic unpredictable mild stress （CUMS） , then they were randomly divided into 5 groups：depressive model group, sulfur lamp group, heat radiation lamp group, fluorescent lamp group and LED lamp group. After 45 d of continuous illumination, serum was collected and reproductive organs were removed from rats, then the organ index of testicle, epididymis, seminal vesicle and kidney was calculated, the concentrations of testosterone （ T）, estradiol （ E2）, prolactin （PRL） and progesterone （PROG） in the serum were detected by ELISA, the histomorphological lesion of testicle was observed under microscope with hematoxylin-eosin （HE） staining, the expression of melatonin receptors （Mel la）, 3β-HSD and P450scc of testicle were detected by Western blot. Results The organ index of testicle, epididymis and seminal vesicle, the concentration of T, E2,PRL and PROG, and the expression of Mel 1 a, 3β-HSD and P450scc in the depressive rats were significantly lower than that of the controls （P 〈 0. 05 ） , and the seminiferous tubules of the testis of depressive rats were atrophied. However, the level of T, E2, PRL and PROG were increased,the cell morphology of the testicle was improved, and the expression of Mel la, 3 β-HSD and and P450scc were upregulated after the sulfur lamp lighting in the depressive male rats compared with depressive model group （ P 〈 0.05 ）. Conclusions The testis structure and functions of depressive male rats are improved by sulfur lamp lighting.

关 键 词：雄性抑郁大鼠 生殖 微波硫灯 睾酮 

分 类 号：R749.4[医药卫生—神经病学与精神病学]