低聚壳聚糖衍生物与BSA的作用研究  

Interaction between Low Molecular Weight Chitosan Derivatives and Bovine Serum Album

作　　者：黄燕[1] 刘云[1] 张岐[1,2] 贾春满 华明清 曹娟[1] 朱卫华[1] 

机构地区：[1]江苏大学化学化工学院,江苏镇江212013 [2]海南精细化工重点实验室,海南海口570228

出　　处：《光谱学与光谱分析》2017年第6期1814-1818,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21601068);江苏省博士后科研资助计划项目(1401050C);江苏省高校自然科学研究面上项目(14KJB150006);江苏大学高级人才科研启动基金项目(14JDG054)资助

摘　　要：研究了具有生物相容性的低聚壳聚糖衍生物与活性药物牛血清白蛋白(BSA)之间的作用。合成了聚合度为20的窄分子量分布低聚壳聚糖(NLCS_(20))的3种衍生物:N-马来酰化低聚壳聚糖(MNLCS)、N-羧甲基低聚壳聚糖(CNLCS)和N-琥珀酰化低聚壳聚糖(SNLCS)。通过紫外光谱(UV)、荧光光谱和等温滴定微量热仪(ITC)研究了NLCS_(20)衍生物与BSA之间的相互作用。结果表明,BSA的构象在NLCS_(20)衍生物溶液微环境中没有发生变化,活性也没有受到影响。NLCS_(20)衍生物与BSA的作用过程是放热的且是自发进行的,主要作用力是氢键和疏水作用。说明低聚壳聚糖衍生物能够作为潜在的亲水药物如活性医药蛋白或氨基酸的运输载体。The effect between biocompatible low molecular weight chitosan derivatives as the delivery carrier of active pharmaceuticals bovine serum album （BSA） was studied. Three derivatives of low molecular weight chitosan with 20 degree of polymerization （NLCS20） and narrow molecular weight distribution including N-maleoyl-NLCS20, N-carboxymethyl-NLCS20 and N-succinyl-NLCS20 were synthesized. The effect between NLCS20 derivatives and BSA was studied with UV spectrum, fluorescence spectrum and isothermal titration calorimetry （ITC）. The results showed that the conformation and activity of BSA did not change a lot in the microenvironment of NLCS20 derivatives solution. The process was exothermic and spontaneous. The main binding force was hydrogen bond and hydrophobic interaction. It was concluded that low molecular weight derivatives are likely to be delivery carriers of hydrophilic drugs such as active protein or poly amino acid.

关 键 词：低聚壳聚糖 衍生物 牛血清白蛋白 作用 

分 类 号：O636.1[理学—高分子化学]