基于脾主长夏探讨健脾益智胶囊对AD大鼠TChE、GFAP表达的影响  

Exploration on the Effects of Jianpi Yizhi Capsule on the Expression of TCHE and GFAP in the Rats with Alzheimer Disease Based on the Spleen Governing the Last Month of Summer

作　　者：刘启鸿[1] 李晓明[1] 程燕彬 杨光[1] 杜杰勇 蔡华珠[1] 

机构地区：[1]福建中医药大学中医学院,福州350122

出　　处：《中国中医药现代远程教育》2017年第9期128-130,共3页Chinese Medicine Modern Distance Education of China

基　　金：国家自然科学基金资助项目【No.81202613】;国家级大学生创新训练计划项目【No.20141039001】;省级大学生创新训练计划项目【No.20151039057】

摘　　要：目的探讨健脾益智胶囊对阿尔茨海默病(AD)大鼠的作用机理。方法通过腹腔注射D-半乳糖,同时胃饲氯化铝(ALCL_3)的方法塑造AD动物模型,设立空白对照组、模型组、长夏组、非长夏组,采用酶联免疫法(Elisa)法检测其尾血血清中乙酰胆碱酯酶TChE,应用免疫组化法测定给药后AD大鼠海马区胶质纤维酸性蛋白(GFAP)的阳性细胞数。结果给药后,与非长夏组、模型组比较,长夏组TChE活性明显降低(P<0.01)以及GFAP表达明显升高(P<0.05或P<0.01)。结论健脾益智胶囊能够抑制TChE活性,间接提高血清中乙酰胆碱的含量,并通过升高大鼠海马区GFAP阳性表达,促进星形胶质细胞的激活,达到治疗AD的作用,从而验证"脾主长夏"理论的科学性。Objective To explore the mechanism of Jianpi Yizhi capsule in treatment of Alzheimer＇s disease of rats. Methods The AD rats model was established by D（ ＋） Galactose injected into the rats and intragastric administration of ALCL3. The rats were classified into 4 groups as follows： blank control group, model group, zhangxia group and non-zhangxia group. ELISA method was used to measure the tail serum blood acetylcholinesterase（ TChE）, and GFAP expression of astrocytes in hippocampal region was measured with immunohistochemical staining. Results Comparing with non-zhangxia group and the model group, TChE in zhangxia group was decreased（P〈0.01）, while the number of GFAP positive cell significantly increased（P〈0.05 or P〈0.01） after asminister drugs. Conclusion Jianpi Yizhi capsule can suppress the activity of TChE, increase the contents of Ach in rats indirectly, and improve the expression of GFAP in hippocampal region, promote activation of astrocytes in the AD rats, it has the therapy effect on the treatment of AD that verified the scientific theory of ＂ spleen governing the last month of summer＂.

关 键 词：脾主长夏 健脾益智胶囊 阿尔茨海默病 乙酰胆碱酯酶 胶质纤维酸性蛋白 呆证 动物实验 

分 类 号：R285.5[医药卫生—中药学]