高效液相色谱串联质谱同时测定猪肉中克仑特罗、沙丁胺醇含量的方法学考察  

The Methodology of HPLC-Ms Method for Simultaneous Determination of Clenbuterol and Salbutamol in Pork

作　　者：郭丽娜[1] 王蕾[1] 金善玉[1] 

机构地区：[1]延边州食品药品检验所食品理化室,吉林延吉133000

出　　处：《中国卫生标准管理》2017年第11期12-14,共3页China Health Standard Management

摘　　要：目的建立高效液相色谱串联质谱同时测定猪肉中克仑特罗和沙丁胺醇含量的方法学考察。方法以克仑特罗-D9、沙丁胺醇-D3为内标物,选择离子对为克仑特罗m/z 277→m/z 203;沙丁胺醇m/z 240→m/z 148;克仑特罗-D9 m/z 280→m/z 204;沙丁胺醇-D3m/z 243→m/z 151。结果克仑特罗和沙丁胺醇的精密度和重复性良好、线性范围分别为13.18~79.08 ng/ml,9.85~59.10 ng/ml且回收率良好。结论建立的方法准确且灵敏度高,适用于克仑特罗和沙丁胺醇的残留量测定。Objective To develop a HPLC-Ms method for simultaneous determination of clenbuterol and salbutamol in pork. Methods Clenbuterol-D9 and Salbutamol-D3 were used as internal standard and the ion pair for Clenbuterol m/z 277→m/z 203; Salbutamol m/z 240 → m/ z 148; Clenbuterol-D9 m/z 280 → m/z 204; Salbutamol-D3 m/z 243 → m/Z 151. Results The precision and reproducibility of clenbuterol and salbutamol were good, the linear range was 13.18 to 79.08 ng/ml, 9.85 to 59.10 ng/ml and the recovery rate was good. Conclusion This method is accurate and sensitive and it is suitable for the determination of the amount of Salbutamol and Clenbuterol residues.

关 键 词：高效液相色谱串联质谱 克仑特罗 沙丁胺醇 方法学 

分 类 号：S859.84[农业科学—临床兽医学]