脓毒症患儿CD14^＋单核细胞中miR-146a的表达及其临床意义  

Expression and clinical significance of miRNA-146a in CD_(14)^+ MC of children with sepsis

作　　者：武宇辉[1] 齐颖[1] 马伟科[1] 李雨铮[1] 杨卫国[1] 李成荣[1] 

机构地区：[1]广东省深圳市儿童医院PICU,广东深圳518038

出　　处：《实用临床医药杂志》2017年第9期119-122,共4页Journal of Clinical Medicine in Practice

基　　金：广东省深圳市科技计划项目(JCYJ20140416141331523)

摘　　要：目的观察儿童脓毒症患者CD_(14)^+单核细胞(MC)中miR-146a的表达水平。方法选取40例脓毒症患儿。选择15例手术后全身性炎症反应综合征(SIRS)患儿和15例健康儿童作为对照组。通过实时荧光定量PCR(qRT-PCR)方法检测CD_(14)^+MC中miR-146a水平和肿瘤坏死因子-α(TNF-α)、白细胞介素10(IL-10)的表达水平。流式细胞仪检测CD_(14)^+MC HLA-DR水平。测定外周血超敏C反应蛋白(hs-CRP)、降钙素原(PCT)水平。结果脓毒症患儿CD_(14)^+MC的HLA-DR水平均>30%。脓毒症患儿CD_(14)^+MC的miR-146a表达水平较SIRS组和健康对照组显著升高(P<0.05),在恢复期其水平显著下调(P<0.05)。脓毒症患儿CD_(14)^+MC的TNF-α、IL-10水平均较健康对照组显著升高(P<0.05)。结论脓毒症患儿CD_(14)^+MC中miR-146a表达上调。Objective To investigate the expression of miR-146a in CD14 ^＋ monocytes （MC） of children with sepsis. Methods Forty children with sepsis in PICU were enrolled in the study. Fifteen children with systemic inflammatory response syndrome （SIRS） after surgical treatment and fifteen healthy children were selected as control group. Expressions of miR-146a and TNF-α, IL-10 of CD14^＋ MC were detected by real - time quantitative PCR （qRT-PCR）. HLA-DR levels of CD14^＋ MC were detected by flow cytometry. Levels of high - sensitivity C-reactive protein （CRP） and procalcitonin （PCT） were detected. Results The HLA-DR levels of CD14^＋ MC in all the children with sepsis were over 30%. The miR - 146a level of CD14^ ＋ MC were significantly higher in sepsis group than SIRS group and healthy controls （P 〈 0.05 ） , and the level decreased when sepsis children were recovered （P 〈 0.05 ）. The TNF-α, IL-10 level of CD14 ^＋ MC in children with sepsis was significantly higher than healthy controls （P 〈0.05）. Conclusion The expressions of CD14 ^＋ MC miR-146a in children with sepsis are significantly up-regulated.

关 键 词：儿童 微小RNA-146a 脓毒症 CD14^+单核细胞 

分 类 号：R631[医药卫生—外科学]