光甘草定对人皮肤成纤维细胞的作用研究  

Effects of phytoestrogen Glabridin on human skin fibroblasts in vitro

作　　者：王子偲 顾怀宇[1] 

机构地区：[1]中山大学中山医学院解剖学与神经生物学教研室,广州510080

出　　处：《中国医学前沿杂志（电子版）》2017年第4期22-26,共5页Chinese Journal of the Frontiers of Medical Science(Electronic Version)

基　　金：广东省教育部科技部产学研结合项目(2012B090600019)

摘　　要：目的探讨光甘草定对人皮肤成纤维细胞的作用。方法将体外培养的人皮肤成纤维细胞分为空白对照组(仅加入单细胞悬液)、阳性对照组(10 nmol/L的17β-雌二醇处理)及实验组(光甘草定浓度分别为:0.1、1、10、100μmol/L),作用72小时后,采用活细胞计数试剂盒-8(CCK-8)检测法、5-乙炔基-2'-脱氧尿苷(5-ethynyl-2'-deoxyuridine,EdU)掺入法检测成纤维细胞增殖活性;流式细胞术检测细胞S期所占比例。结果 10μmol/L实验组和阳性对照组的光密度均明显高于空白对照组(P<0.05),100μmol/L实验组光密度明显低于空白对照组(P<0.05),但10μmol/L实验组和阳性对照组光密度比较无显著差异(P>0.05)。10μmol/L实验组和阳性对照组的EdU标记的阳性细胞百分比明显高于空白对照组(P<0.05),但10μmol/L实验组和阳性对照组EdU标记的阳性细胞百分比无显著差异(P>0.05)。10μmol/L实验组和阳性对照组的S期占比明显高于空白对照组(P<0.05),但10μmol/L实验组和阳性对照组S期占比无显著差异(P>0.05)。结论光甘草定可以促进人皮肤成纤维细胞的增殖。Objective This study was designed to elucidate the effects ofphytoestrogen Glabridin on the proliferation of human skin fibroblast （HSF）. Method HSFs were divided into blank control group （only single cell suspension was added）, positive control group （treated by 10 μmol/L 17β-estradiol） and experience group （0.1, 1, 10 and 100 μmol/L Glabridin）. After 72 hours, the Cell Counting Kit-8 （CCK-8） assay and 5-ethynyl-2＇-deoxyuridine （EdU） incorporation assay were performed to assess cell viability, and the phase of cell generation cycle was determined by flow cytometry. Result The optical density （OD） of 10 μmol/L experimental group and positive control group were significantly higher than that of blank control group （P 〈 0.05）; OD of 100 I.tmol/L experimental group was significantly lower than that of blank control group （P 〈 0.05）, but there was no significant difference in OD between 10 μmol/L experimental group and positive control group （P 〉 0.05）. The percentage of EdU labeled positive cells in 10 μmol/L experimental group and positive control group were significantly higher than that in blank control group （P 〈 0.05）, but there was no significant difference in percentage of EdU labeled positive cells between 10 μmol/L experimental group and positive control group （P 〉 0.05）. The proportion of S phase in 10 μmol/L experimental group and positive control group were significantly higher than that of blank control group （P 〈0.05）, but there was no significant difference in proportion of S phase between 10 μmol/L experimental group and positive control group （P 〉 0.05）. Conclusion Glabridin promote the proliferation of HSF.

关 键 词：植物雌激素 光甘草定 人皮肤成纤维细胞 细胞增殖 

分 类 号：R285[医药卫生—中药学]