类风湿关节炎患者外周血肿瘤坏死因子受体相关因子1表达研究  

Expression of tumor necrosis factor receptor-associated factor 1 in rheumatoid arthritis

作　　者：程韬[1] 耿德春[2] 王鸣军[1] 陈志伟[1] 张缪佳[3] 武剑[1] 

机构地区：[1]苏州大学附属第一医院风湿免疫科,江苏苏州215006 [2]苏州大学附属第一医院骨科,江苏苏州215006 [3]江苏省人民医院风湿免疫科,江苏南京210029

出　　处：《中国实用内科杂志》2017年第6期529-531,共3页Chinese Journal of Practical Internal Medicine

基　　金：江苏省临床医学科技专项类风湿关节炎分子分型诊断研究(BL2013034);苏州市科技局科技发展计划(SYSD2014078);苏州大学青年教师自然科学基金(SDY2013A28)

摘　　要：目的检测类风湿关节炎(RA)患者血清肿瘤坏死因子受体相关因子1(TRAF1)水平,并分析其与RA疾病活动性关系,探讨其与CD40在RA发病机制中的共同作用。方法采集2015年7月至2015年12月苏州大学附属第一医院门诊及住院部70例初诊RA患者和50名健康体检者外周血,记录RA患者临床及实验室指标,酶联免疫吸附法(ELISA)检测TRAF1、CD40,并分析两者相关性。结果 RA患者TRAF1、CD40分别为(35.88±51.22)ng/L,(72.70±70.56)ng/L,与健康对照组[TRAF1和CD40分别为(12.51±8.59)ng/L和(31.54±27.11)ng/L]相比差异均有统计学意义(P<0.05)。根据DAS28评分,将RA患者分为高、中、低活动期组,3组TRAF1分别为(13.05±4.29)ng/L、(29.84±42.48)ng/L和(58.84±66.90)ng/L,差异有统计学意义(F=6.382,P=0.003),且TRAF1与DAS28评分呈正相关(r=0.419,P<0.001)。RA患者血清TRAF1与自身抗体RF、GPI呈正相关(r=0.365,P=0.001;r=0.767,P<0.001)。RA患者血清TRAF1表达与CD40呈正相关(r=0.465,P<0.001)。结论 TRAF1在RA患者明显增高,与RA疾病活动性、自身抗体和CD40呈正相关。Objective To determine levels of tumor necrosis factor receptor-associated factor 1 （TRAF1） in rheumatoid arthritis （RA） patients, and to assess the relationship of TRAF1 levels with autoantibodies, disease activity and CD40. Methods Serum was obtained from 79 RA patients and from 50 healthy individuals. Levels of TRAF1 and CD40 were measured by enzyme-linked immune sorbent assay （ELISA） and the relationship between them was analyzed. Results We found that serum level ofTRAF 1 and CD40 was （35.88±51.22）ng/L and （72.70±70.56）ng/L, respectively; which were significantly higher than controls. Patients were divided into three groups according to DAS28, low disease activity group, moderate disease activity group and high disease activity group. The differences of TRAF1 levels in these three groups were significant （F=6.382, P=0.003）. We also found that the numerical DAS28 had a significant positive correlation with TRAF1 level （r=-0.419, P〈0.001）. Correlation analysis showed that serum levels of TRAF1 were positively associated with RF （r=0.365, P=0.001）, GPI（r=0.767, P〈0.001） and CD40（r=0.465, P〈0.001）. Condusion The serum level of TRAF1 is significantly higher in RA patients and has a significant ly positive correlation with disease activity, autoantibody and CD40.

关 键 词：肿瘤坏死因子受体相关因子1 关节炎 类风湿 自身抗体 

分 类 号：R593.22[医药卫生—内科学]