新城疫病毒M基因片段的表达纯化及其多克隆抗体的制备  

Expression and purification of the matrix gene fragment of Newcastle disease virus and its polyclonal antibody preparation

作　　者：曲永星 王新[2] 詹媛[3] 茅翔[3] 丁铲[3] 唐兆新[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]南京农业大学动物医学院,江苏南京210095 [3]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国兽医科学》2017年第6期745-749,共5页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31572585);国家自然科学基金面上项目(31372421)

摘　　要：M蛋白是新城疫病毒(NDV)的一个多功能结构蛋白。为了制备针对M蛋白的多克隆抗体,本研究通过克隆M蛋白部分基因片段至原核表达载体,重组蛋白在大肠杆菌中表达后进行了纯化。免疫新西兰大白兔获得血清并纯化后得到抗M蛋白多克隆抗体。Western-blot分析结果显示,该抗体具有良好的特异性。本试验成功制备的抗M蛋白多克隆抗体,为进一步研究M蛋白的生物学功能奠定了良好的基础。Matrix protein of Newcastle disease virus is a multifunctional protein. In order to prepare its polyclonal antibodies against matrix protein,we cloned a fragment of matrix gene into prokaryotic expression vector,and the recombinant protein was expressed in Escherichfa colf and purified. New Zealand white rabbit was immunized with recombinant protein to obtain anti-M serum. The polyclonal antibodies was purified. Western-blot analysis showed that the polyclonal antibodies had good specificity against matrix protein. The polyclonal antibodies is useful for further research on biological functions of matrix protein.

关 键 词：新城疫病毒 原核表达 M蛋白 多克隆抗体 

分 类 号：S852.659.5[农业科学—基础兽医学]