丛枝菌根真菌(AMF)对盐胁迫下芦笋植株渗透调节物质及抗氧化酶活性的影响  

Effects of Arbuscular Mycorrhiza Fungi (AMF) on Osmoregulation Substances and Antioxidant Enzyme Activities of Asparagus Plant Under Salt Stress

作　　者：曹岩坡[1] 代鹏[1] 戴素英[1] 

机构地区：[1]河北省农林科学院经济作物研究所,石家庄050051

出　　处：《西南大学学报（自然科学版）》2017年第5期43-48,共6页Journal of Southwest University(Natural Science Edition)

基　　金：河北省科技支撑计划(17226913D);河北省农业创新工程(F17R07)

摘　　要：以芦笋品种‘NJ978’为材料,研究了丛枝菌根真菌(AMF)对NaC1胁迫下芦笋幼苗生长、渗透调节物质及抗氧化酶活性的影响.结果表明:在非盐条件下,接种AMF可以显著促进芦笋植株的生长,提高拟叶可溶性蛋白质量分数、SOD、POD和CAT活性.在NaC1胁迫下,芦笋幼苗生长受到严重抑制,株高、地上部和地下部鲜质量、干质量均显著降低,接种AMF可以有效缓解盐胁迫对芦笋幼苗生长的抑制.在盐胁迫下,芦笋拟叶可溶性蛋白质量分数、MDA质量分数、电解质渗透率和POD活性均比对照显著升高,SOD和CAT活性显著降低;接种AMF可以显著提高盐胁迫下芦笋拟叶可溶性蛋白质量分数、SOD、POD和CAT活性,而显著降低MDA质量分数和电解质渗透率.由此表明,盐胁迫下接种AMF可通过促进芦笋体内渗透调节物质积累和提高抗氧化酶活性,有效降低细胞膜脂过氧化水平,从而缓解盐胁迫对植株的伤害.The effects of arbuscular mycorrhiza fungi （AMF） on osmoregulation substance contents and an tioxidant enzyme activities of asparagus variety‘NJ978’ under salt stress were studied in a greenhouse. Under non-salt stress conditions, AMF inoculation significantly promoted the growth of the asparagus plants and increased soluble protein contents and SOD, POD and CAT activities in their leaf-like blades. Compared with the control, NaCl treatment significantly decreased the height, fresh and dry weight, and SOD and CAT activities in the asparagus plants; after AMF inoculation, the inhibitory effects of salt stress on the plants were effectively alleviated. Under salt stress, soluble protein contents, MDA contents, electrolyte leakage rate and POD activity in the asparagus plants were significantly higher and SOD and CAT activities were lower than those in the control; and AMF inoculation significantly increased their soluble protein contents and SOD, POD and CAT activities, and decreased their MDA contents and electrolyte leakage rate. The above results indicated that AMF inoculation could promote the accumulation of soluble protein, raise the activities of antioxidant enzymes, decrease the membrane lipid peroxidation and improve the adaptability of asparagus plants under salt stress.

关 键 词：芦笋 盐胁迫 丛枝菌根 渗透调节 抗氧化酶 

分 类 号：S644.6[农业科学—蔬菜学]