12株牛源肺炎克雷伯菌ESBLs类抗生素的耐药性与耐药基因检测  

ESBLs Antibiotic Resistance Analysis and Resistance Gene Detection of 12 Strains of Klebsiella pneumonia from Bovine

作　　者：刀丽梅 韩冠双 曹立亭[1] 程方俊[1] 黄攀[1] 郭建华[1] 方志超[1] 林雅[1] 

机构地区：[1]西南大学(荣昌校区)动物医学系,重庆荣昌402460

出　　处：《西南大学学报（自然科学版）》2017年第5期49-53,共5页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市重点科技攻关项目(CSTC2012jcsf-jfzhX0021);西南大学博士启动基金(2013BSr03)

摘　　要：为了解重庆地区12株牛源肺炎克雷伯菌的超广谱β-内酰胺酶耐药性及ESBLs基因型分布情况,该试验采用纸片扩散法对12株肺炎克雷伯菌进行超广谱抗菌药物的敏感性检测,通过PCR方法检测其所产生的耐药基因类型.结果表明:12株菌对阿莫西林、氨苄西林的耐药率均为100%,头孢噻吩和先锋霉素Ⅴ的耐药率为17%,头孢曲松的耐药率为0%.产ESBLs菌株3种耐药基因TEM,SHV,CTX-M的检出率分别为100%,42%,67%,以TEM的检出率最高.The purpose of this study was to detect the drug resistance and the genotypes of extended-spec- trum β-1actamases （ESBLs） of 12 strains of Klebsiella pneumonia, which were isolated from bovine in Chongqing. The Kirby-Bauer （K-B） method was employed to determine the ultra-broad spectrum antimi- crobial sensitivity of these K. pneumonia strains, and PCR was performed to detect the type of resistance genes in them. The results indicated that all the K. pneumonia strains showed resistance to amoxicillin and ampicillin, and 17% of them were resistant to cefalotin and cephalothin V, while no strains could endure ceftriaxone. The positive rate of the three resistance genes, TEM, SHV and CTX-M of ESBLs, in the strains were 100%, 42% and 67%, respectively.

关 键 词：牛源肺炎克雷伯菌 超广谱Β-内酰胺酶 药物敏感性 耐药基因 

分 类 号：S855.1[农业科学—临床兽医学]