胆固醇代谢紊乱SD大鼠肝细胞膜生物泵糠蛋白表达的平行对照分析  

Parallel control analysis of Biological pump glycoprotein expression of SD rat liver cell membrane with cholesterol metabolism disorder

作　　者：余静喜 

机构地区：[1]广东省惠州市第三人民医院普外科,广东惠州516002

出　　处：《中国医药科学》2017年第7期9-11,30,共4页China Medicine And Pharmacy

基　　金：广东省惠州市科技计划项目(2016Y162)

摘　　要：目的分析胆固醇代谢紊乱的SD大鼠肝细胞膜生物泵糖蛋白(Pgp)及相关生化指标与正常大鼠的差异性。方法选取SD大鼠30只,随机均分为对照组与观察组,每组各15只;观察组大鼠以高脂饲料喂养法制备为胆固醇代谢紊乱模型,对照组大鼠常规喂养保持健康;以免疫组化法分析两组大鼠肝脏Pgp表达水平并进行比较,同时比较两组大鼠肝匀浆的各项相关生化指标。结果对照组大鼠肝脏Pgp平均光度值为(1.01±0.13),肝指数为(1.69±0.68);观察组大鼠肝脏Pgp平均光度值为(5.36±1.21),肝指数(3.35±0.37);观察组肝脏Pgp平均光度值与肝指数均显著高于对照组(P<0.01)。观察组肝脏脂肪病变+++的发生率为53.33%,对照组无+++级别肝脏脂肪病变发生,观察组整体肝脏脂肪病变程度较对照组严重(P<0.01)。观察组与对照组大鼠初始体质量、肝匀浆TBA,差异无统计学意义(P>0.05);观察组与对照组大鼠血清Tch比较差异有统计学意义(P<0.05);两组其他各项指标均差异具有统计学意义(P<0.01)。肝脏脂肪病变程度与肝脏Pgp光度值呈显著高度正相关性,0.8<r<1,P<0.01。结论胆固醇代谢紊乱SD大鼠的肝细胞膜生物泵糖蛋白表达显著高于健康大鼠,提示肝脏Pgp过高表达在胆固醇代谢紊乱的发生发展过程中发挥着重要作用,应引起胆固醇代谢紊乱防治研究的关注。Objective To analyze the difference of Biological pump glycoprotein （Pgp） of SD rat liver cell membrane with cholesterol metabolism disorder and its related biochemical indexes and the normal rats. Methods 30 SD rats were selected and randomly divided into observation group and control group,15 rats in each group.The rats in observation group fed with high fat diet were prepared for the disorder of cholesterol metabolism in model,and rats of control group fed heahhy.Immunohistochemical method was used to analyze the expression of Pgp in two groups of rats. And they were compared.The related biochemical indexes of rat liver homogenate of the two groups were also compared. Results The average Pgp value of rat liver of control group was （1.01 ＋ 0.13）,the liver index was （1.69 ± 0.68）.The average Pgp value of rat liver of observation group was （5.36 ± 1.21）,the liver index was （3.35± 0.37）. The average Pgp value of rat liver and liver index in observation group group were significantly higher than those of control group （P 〈 0.01）.The hepatic steatosis ＋＋＋ incidence rate of observation group was 53.33%,the control group had no pathological changes of liver fat level ＋＋＋.The observation group overall hepatic steatosis degree was more serious than the control group, （P 〈 0.01）.There were no statistical differences on rats initial body weight and liver homogenate TBA between the observation group and the control group （P 〉 0.05）.There was some statistical difference on rats serum Tch between observation group and control group（P 〈 0.05）.The other indexes showed statistically significant differences,（P 〈 0.01）.There was a significant positive correlation between the degree of hepatic steatosis and Pgp value of liver,0.8 〈 r 〈 I,P 〈 0.01. Conclusion The liver cell membrane glycoprotein biological pump cholesterol metabolism in SD rats is significantly higher than that of healthy rats,which suggests that liver Pgp overexpression plays an important rol

关 键 词：胆固醇代谢紊乱 P-糖蛋白 肝脏 内分泌 

分 类 号：R259[医药卫生—中西医结合]