草地早熟禾PpGA2ox基因的克隆、亚细胞定位及表达分析  

Cloning,Subcellular Localization and Expression Analysis of PpGA2ox Gene in Kentucky Bluegrass

作　　者：檀鹏辉 李俊[2] 陈小云 张兰[1] 滕珂[1] 尹淑霞[1] 

机构地区：[1]北京林业大学林学院草坪研究所,北京100083 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010 [3]深圳市国艺园林建设有限公司,深圳518038

出　　处：《中国草地学报》2017年第3期8-14,30,共8页Chinese Journal of Grassland

基　　金：国家自然科学基金项目"空间诱变草地早熟禾矮化突变体矮化机理研究"(31302016);深圳市科技计划项目"海绵型城市绿地建植关键技术研发"(JSGG20160229155434792)资助

摘　　要：利用RACE技术从草地早熟禾中克隆赤霉素合成代谢的关键酶GA2-氧化酶基因(PpGA2ox),提交到GenBank,登录号为KX254272。其开放阅读框为1047bp,编码348个氨基酸。系统进化分析表明,PpGA2ox与大麦、小麦及二穗短柄草中的GA2ox亲缘关系较近。亚细胞定位结果显示,其编码的蛋白定位于细胞核和细胞质中。实时荧光定量分析表明,PpGA2ox在幼嫩叶片中的表达量最高,且受GA3、MeJA及IAA三种激素的诱导。To investigate the GA 2-oxidase gene in Kentucky bluegrass, the PpGA 2ox gene was cloned using RACE method in the study. The cDNA sequence was deposited in the NCBI database with accession number KX254272. The open reading frame of PpGA2ox gene was 1047bp, corresponding to 348 amino acids. Phylogenetic analysis exhibited that the PpGA2ox protein was most closely related to those in barley, wheat and Brachypodium distachyon. Subcellular localization results proved that the PpGA2ox pro- tein was localized in both the nucleus and cytoplasm. Real-time quantitative PCR analysis showed that PpGA 2ox gene was expressed most abundantly in young leaves. Moreover, its expression could be induced by exogenous GA3, MeJA or IAA.

关 键 词：草地早熟禾 GA2-氧化酶基因 克隆 亚细胞定位 表达分析 

分 类 号：S688.4[农业科学—观赏园艺]