基于ISSR标记的野生斑茅居群遗传结构研究  

A Study on Genetic Structure of Wild Erianthus arundinaceus Populations Based on ISSR Markers

作　　者：张建波[1] 鄢家俊[1] 白史且[1] 常丹[1] 张瑜[1] 张劲[1] 王毅 

机构地区：[1]四川省草原科学研究院,四川成都611731 [2]雅安市农业科学研究所,四川雅安625000

出　　处：《中国草地学报》2017年第3期23-30,共8页Chinese Journal of Grassland

基　　金：国家自然科学基金项目(31302026);四川省公益性科研院所基本科研项目"能源植物斑茅产能特性评价及新材料创制"(1618);国家现代农业产业技术体系建设专项资金(No.CARS35-35)资助

摘　　要：用7条ISSR引物研究我国18个野生斑茅居群的遗传多样性和遗传结构,结果表明:(1)7条ISSR引物共扩增出69条清晰条带,多态性条带59条,占83%;观测等位基因数、有效等位基因数、Shannon指数和Nei’s基因多样性指数等指标均显示我国斑茅遗传多样性丰富。(2)斑茅居群内的变异大于居群间,由此推测斑茅是异花授粉的地理广布种。(3)聚类分析发现,斑茅居群间存在明显的遗传分化,遗传距离和地理距离的Mantel检测发现两者的相关性弱。(4)斑茅各居群间的基因流较小,地理环境的隔离及生境异质影响到斑茅变异在居群内和居群间的分布、聚类结果及斑茅的居群结构。The genetic structure of 18 wild Erianthus arundinaceus populations from China were ana- lyzed using 7 ISSR markers. The results showed that. 1） 7 ISSR markers generated 69 bands and 83% were polymorphic bands. There was rich genetic diversity among the tested wild resources of Erianthus arundinaceus. 2） The genetic variation within populations was bigger than among populations, which sug- gested that E. arundinaceus was a cross-pollination species. 3） Cluster analysis showed that there was a significant genetic differentiation among populations, the Mantel test of geographic distances and genetic distance showed weak correlation between them. 4） The gene flow among populations was small, which was affected by the isolation of geographical environment and the influence of habitat heterogeneity, and then affected the distribution, clustering results and population structure of the populations.

关 键 词：斑茅 ISSR 遗传结构 聚类分析 Mantel检验 

分 类 号：S543.9[农业科学—作物学]