HPLC测定脑得生固体分散体胶囊中三七皂苷类成分的含量  

Determination of Panax Notoginseng Saponins in Naodesheng Solid Dispersion Capsules by HPLC

作　　者：罗兰[1,2,3] 李明丽[1] 康家珍 王淑美[1,2,3] 梁生旺[1,2,3] 

机构地区：[1]广东药科大学中药学院,广州510006 [2]广东药科大学国家中医药管理局中药数字化质量评价技术重点研究室,广州510006 [3]广东药科大学广东高校中药质量工程技术研究中心,广州510006

出　　处：《海峡药学》2017年第5期43-46,共4页Strait Pharmaceutical Journal

基　　金：国家自然科学基金面上项目(81274059)

摘　　要：目的建立脑得生固体分散体胶囊中三七皂苷类成分(三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1)的含量测定方法。方法采用高效液相法,Hyspersil ODS-2色谱柱(5μm,250×4.6mm),柱温25℃,流动相乙腈-水系统梯度洗脱,流速0.9m L·min-1,检测波长203nm。结果三七皂苷R1在0.464~3.480μg(r=0.9999)范围,人参皂苷Rg1在1.968~14.760μg(r=0.9999)范围,人参皂苷Rb1在1.848~13.860μg(r=0.9999)范围均呈良好的线性关系;平均回收率分别为97.60%,99.22%,100.78%;RSD分别为1.86%,2.44%,1.84%。结论该方法简便、准确、重复性好,可用于该制剂的质量控制。OBJECTIVE To simultaneous determine the contents of notoginsenoside R1 , ginsenoside Rg1 , gin- senoside RbI in Naodesheng solid dispersion capsules by HPLC. METHODS An external standard method by HPLC with Hyspersil ODS-2 C ls column （5 μm ,250mm × 4. 6mm） was used with the column temperature was 25 ℃. The detection wavelength was 203nm. The flow rate was set at 0. 9mL · min^-1 and gradient elution with acetonitrile and H20. RESULTS Linearities of notoginsenoside R1 ,ginsenoside Rg1 and ginsenoside Rb1 were good in the ran- ges of 0. 464 - 3.480 μg （ r = 0. 9999 ）, 1. 968 - 14. 760 μg （ r = 0. 9999 ） and 1. 848 - 13. 860μg （ r = 0. 9999 ）, re- spectively. The average recoveries of notoginsenoside R1, ginsenoside Rg1 and ginsenoside RbI were 97.60%, 99.22% ,100.78% and RSD were 1.86% ,2. 44% ,1.84% ,respectively. CONCLUSION The method is simple, accurate and reproducible, and can be used for quality control of Naodesheng solid dispersion capsules.

关 键 词：脑得生固体分散体 HPLC 三七皂苷R1 人参皂苷RG1 人参皂苷RB1 

分 类 号：R927[医药卫生—药学]