Stella^(TM) 5L内镜灭菌系统对软式胆管镜灭菌效果的研究  

Evaluation of Stella^(TM) 5 L sterilization system on microbicidal efficacy of flexible choledochoscope

作　　者：王彩霞[1] 陈圆圆[1] 李蒙[1] 杨帆[1] 李春雨[1] 范玲[2] 

机构地区：[1]中国医科大学盛京医院内镜中心,辽宁沈阳110004 [2]中国医科大学盛京医院护理部,辽宁沈阳110004

出　　处：《实用临床医药杂志》2017年第10期33-35,共3页Journal of Clinical Medicine in Practice

摘　　要：目的采用Stella^(TM)5 L型内镜灭菌系统对软式胆管镜进行灭菌,评价其灭菌效果。方法将三条软式胆管镜表面和管道用枯草杆菌黑色灭种芽孢(ATCC9372)悬液污染,一条镜子直接对表面和管道采样进行活菌计数;另外两条内镜首先进行手工清洗,其中一条镜子对表面和管道采样进行活菌计数,另一条内镜再采用Stella^(TM)5L型内镜灭菌系统进行灭菌处理,然后对表面和管道进行活菌计数。试验重复20个周期,评价Stella^(TM)5L型内镜灭菌系统对细菌减少和杀灭作用。结果枯草杆菌黑色变种芽孢(ATCC9372)悬液污染的软式胆管镜管道,污染后从内镜表面和管道采样的活菌计数分别为(7.10×10~6±0.81×10~6)cfu/m L和(7.11×10~6±1.33×10~6)cfu/m L,单纯经手工清洗后,内镜表面和管道活菌计数分别为(4.82×10~2±0.91×10~2)cfu/m L和(3.15×10~3±3.24×10~2)cfu/m L。而清洗后采用Stella ^(TM) 5L型内镜灭菌系统灭菌处理,20个周期内镜表面和管道均无任何细菌生长。结论 Stella^(TM)5 L型内镜灭菌系统有较强的灭菌作用,且作用时间短,无醛类等任何污染,对人无伤害,适用于软式胆管镜的灭菌。Objective To explore the microbicidal efficacy of StellaTM5 L sterilization system for flexible choledochoscope. Methods The surfaces and working channels of 3 cholangioscopes were contaminated with suspensions of Bacillus atrophaeus （ATCC9372）. Among the three scopes, one scope was randomly selected to count the bacterial load in the recoveries from the surface and chan- nel. The other two endoscopes were manually cleaned and rinsed. One scope was randomly selected to count bacterial presence on the surface and working channel, and the rest endoscope was sterilized by a StellaTM5L endoscopic sterilization system, and then bacterial count of the surface and working channel was performed. The process was repeated for 20 cycles to evaluate the microbicidal efficacy of the StellaTM5L endoscopic sterilization system. Results From an initial contamination level of （7.10 ×10^6 ±0.81 ×10^6） cfu/mL, （7.11 ×10^s ± 1.33 ×10^6） cfu/mL on the surface and channel of the chlangioscopes, respectively, bacterial count was reduced to（4.82 ×10^2 ± 0.91 ×10^2） cfu/mL, （3.15 ×10^3 ± 3.24 ×10^2 ） cfu/mL, respectively, after manual cleaning, but no micro - organisms were discovered in 20 cycles with the StellaTM5L system. Conclusion This study shows that StellaTM 5 L system is capable of delivering the complete elimination of contaminating micro - organisms in a reduced time and eliminates the toxic risk of reprocessing associated with Chlorine based disinfect- ants.

关 键 词：软式胆管镜 灭菌 枯草杆菌黑色变种芽孢 

分 类 号：R472.1[医药卫生—护理学]