尖裸鲤病变死亡后微生物群落结构变化分析  

Changes of Microbial Community after Oxygymnocypris stewarti Death

作　　者：陈美群[1] 李宝海[2] 周建设[1] 潘瑛子[1] 扎西拉姆[1] 王万良[1] 

机构地区：[1]西藏自治区农牧科学院水产研究所,西藏拉萨850032 [2]西藏自治区农牧科学院,西藏拉萨850032

出　　处：《西南农业学报》2017年第5期1233-1238,共6页Southwest China Journal of Agricultural Sciences

基　　金：西藏自治区自然科学基金(13-44);农业公益性行业科研专项(201403012-05)

摘　　要：【目的】本文探讨了尖裸鲤因水霉菌病变死亡后的微生物群落结构变化特征。【方法】采用Illumina-Miseq高通量测序技术对尖裸鲤健康个体和因水霉菌病变死亡个体的表皮皮肤粘液及肠道内容物的微生物多样性进行分析。【结果】尖裸鲤因水霉菌病变死亡后其表皮皮肤和肠道中细菌群落多样性减少,而其真菌多样性增加。尖裸鲤病变死亡后其表皮皮肤粘液中Pseudomonas sp.、Vagococcus sp.、Providencia sp.、Morganella sp.、Pleosporales、Mucorales、Tremellales和Agaricomycetes等8种优势微生物菌群减少,而Acinetobacter sp.、Proteus sp.、Carnobacterium sp.和Malassezia sp.等4种优势微生物菌群增加。同时,尖裸鲤病变死亡后其肠道中Acinetobacter sp.、Flavobacterium sp.、Vagococcus sp.、Carnobacterium sp.、Bacillus sp.和Malassezia sp.等6种优势微生物菌群减少,而Pseudomonas sp.、Tremellales和Agaricomycetes等3种优势微生物菌群增加。【结论】细菌和真菌的群落结构变化是尖裸鲤病变死亡的主要原因。[ Objective ] The present paper aims to explore the changes of microorganisms community between healthy individuals and diseased individuals of Oxygymnocypris stewarti. [ Method ] The skin mucus and intestinal contents of Oxygymnocypris stewarti by using Illumina-Miseq high-throughput sequencing technology were studied. [ Result ] The bacterial diversity in the skin mucus and intestinal contents after Oxygym- nocypris stewarti death were decreased, however, the fungi diversity were increased; The dominant microbial of Pseudomonas sp. , Vagococ- cus sp. , Providerwia sp. , Morganella sp. , Pleosporales, Mucorales, Tremellales and Agaricomycetes in the skin mucus were decreased, meanwhile the dominant microbial of Acinetobacter sp. , Proteus sp. , Carnobacterium sp. and Malassezia sp. were increased; The dominant microbial of Acinetobacter sp. , Flavobacterium sp. , Vagococcus sp. , Carnobacterium sp. , Bacillus sp. and Malassezia sp. in the intestinal contents were decreased, meanwhile the dominant microbial of Pseudomonas sp. , Tremellales and Agaricomycetes were increased. [ Conclusion] The bacterial and fungal community structure changes were the main causes of Oxygymnocypris stewarti, death.

关 键 词：高通量测序 尖裸鲤 微生物群落 水霉病 

分 类 号：S965.116[农业科学—水产养殖]