瘦素受体基因Gln223Arg位点多态性与哮喘和代谢综合征之间关系的研究  

Associations of polymorphism of leptin receptor gene Gln223Arg with asthma and metabolic syndrome

作　　者：高俊杰[1] 秦苗[2] 徐瑞金[1] 张静[1] 葛云洁[1] GAO Jun-Jie QIN Miao XU Rui-Jin ZHANG Jing GE Yun-Jie(The Cadre Health Care Department, Qingdao Municipal Hospital, Qingdao 266071, China)

机构地区：[1]青岛市市立医院干部保健科,青岛266071 [2]青岛大学附属医院,青岛266002

出　　处：《中国免疫学杂志》2017年第5期660-664,共5页Chinese Journal of Immunology

摘　　要：目的:探讨瘦素受体基因Gln223Arg位点多态性与哮喘和代谢综合征之间的关系。方法:选择120例哮喘病人(A)、92例代谢综合征病人(M)、54例哮喘合并代谢综合征病人(A+M)和81例健康对照者(NC),其中哮喘组根据肺功能进一步分为轻-中度哮喘组和重度哮喘组。应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)方法测定所有受试者瘦素受体基因Gln223Arg位点基因型,ELISA法检测血清瘦素浓度,同时测定所有受试者BMI、血压、肺功能及空腹血糖。结果:(1)代谢综合征组和哮喘合并代谢综合征组AA+AG基因型和A等位基因频率较健康对照组显著性增高(P<0.05);(2)哮喘组中重度持续组AA+AG基因型和A等位基因频率较健康对照组显著增高(P<0.05);(3)AA+AG基因型的血浆瘦素浓度、MBI、收缩压与GG基因型比均显著升高,FEV1%、FEV1/FVC明显下降(P<0.05)。结论:瘦素受体Gln223Arg位点基因多态性与哮喘和代谢综合征具有相关性,其中A等位基因可能通过瘦素抵抗诱导哮喘和代谢综合征的发生,是哮喘和代谢综合征的共同遗传易感因子。Objective：To investigate the relationship of polymorphism of leptin receptor gene Gln223Arg with asthma and metabolic syndrome.Methods： Collected 120 asthma patients,92 metabolic syndrome patients,54 asthma combined metabolic syndrome patients and 81 normal controls.According to the severity,the asthma patients were divided into mild-medium group and severe group.The serum leptin level was measured by ELISA,the genotypes of leptin receptor were analyzed by the method of polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）,and statistcs of each subject’s MBI,blood pressure,lung function and fasting blood glucose were collected.Results： There were significant differences in genotype and allele frequency in leptin receptor gene Gln223Arg between the metabolic syndrome group,asthma combined metabolic syndrome group and normal control group（P 〈0.05）. The allele frequency and genotype in leptin receptor gene Gln223Arg were significant different between the severe asthma group and normal control group（P〈0.05）.The serum leptin level,BMI and systolic blood pressure of AA＋AG genotype group were significiant higher than GG genotype group（P〈0.05）,while the value of FEV1% and FEV1/ FVC of lung function were lower than GG genotype group（P 〈 0.05） .Conclusion： Leptin receptor gene Gln223Arg polymorphism is correlated with asthma and metabolic syndrome,and by causing leptin resistance,the A allele might be the genetic factor that contribute to individual susceptibility for asthma and metabolic syndrome.

关 键 词：哮喘 代谢综合征 瘦素 瘦素受体 基因多态性 

分 类 号：R392.2[医药卫生—免疫学]