Th22细胞及其功能分子IL-22在狼疮性肾炎中的作用研究  

Study on role of Th22 cells and its functional cytokine IL-22 in lupus nephritis

作　　者：顾丽萍[1] 杨贵丽[1] 陈幼发[2] 陶书超[3] GU Li-Ping YANG Gui-Li CHEN You-Fa TAO Shu-Chao(Department of Internal Medicine, Hospital of Wuhan University of Science and Technology, Wuhan 430065, Chin)

机构地区：[1]武汉科技大学医院内科,武汉430065 [2]武汉科技大学附属天佑医院肾病科,武汉430070 [3]枣阳市第一人民医院检验科,枣阳441200

出　　处：《中国免疫学杂志》2017年第5期755-758,共4页Chinese Journal of Immunology

摘　　要：目的:观察狼疮性肾炎(LN)患者外周血中的Th22细胞及其功能分子白细胞介素(IL)-22的表达,并探讨其意义。方法:选择系统性红斑狼疮患者(无肾脏受累)38例,狼疮性肾炎患者32例,健康志愿者10例,分别作为SLE组、LN组和健康对照组(HC组)。应用流式细胞仪检测其外周血中Th22细胞,酶联免疫吸附实验检测患者血清中IL-22的水平。比较三组Th22细胞和血清中IL-22水平差异,分析LN组Th22细胞和IL-22与SLE疾病活动指数评分(SLEDAI)的相关性。结果:SLE组和LN组患者外周血中Th22细胞较健康对照组明显升高(P<0.01),血清中IL-22水平也较健康对照组明显升高(P<0.01);Th22和IL-22在LN组较SLE组要稍微高表达,但差异均无统计学意义(P>0.05);LN组患者Th22细胞比例和IL-22水平与对应患者的疾病活动性评分SLEDAI呈正相关。结论:Th22细胞和其功能分子IL-22参与SLE和LN的发病和进展。Objective：To observe the expressions of Th22 cells and IL-22 in peripheral blood of patients with Lupus nephritis（LN） and explore its significance.Methods： Patients of systemic lupus erythematosus （SLE） with no renal involvement （n = 38）,lupus nephritis （n =32） and healthy controls （n = 10） were studied.Flow cytometry was utilized to quantify the percentage of Th22 cells in peripheral blood.Enzyme-linked immunosorbent assay was used to detect the levels of serum IL-22.The Th22 cells and The serum level of IL-22 among three groups were compared,and the correlation between Th22,IL-22 and SLE disease activity index score （SLEDAI） was analyzed.Results： Th22 cells in SLE group and LN group were significantly higher than those in healthy control group （P〈0.01）.Serum IL-22 level was also significantly higher than that of healthy control group （P〈0.01）;Th22 and IL-22 LN groupwere slightly higher than that in SLE group,but the difference was not significant （P〉0.05）.The proportion of Th22 cells and the level of IL-22 in LN group were positively correlated with the disease activity score SLEDAI.Conclusion： Th22 cells and IL-22 may play an important role in the pathogenesis of SLE and LN.

关 键 词：TH22细胞 IL-22 狼疮性肾炎 

分 类 号：R593.242[医药卫生—内科学]